Optically mapping multiple bacterial genomes simultaneously in a single run

Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demo...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2011-11, Vol.6 (11), p.e27085-e27085
Main Authors: Riley, Matthew C, Lee, James Eric, Lesho, Emil, Kirkup, Jr, Benjamin C
Format: Article
Language:English
Subjects:
Bacteria
Bacterial genetics
Biology
Chromosome Mapping - methods
Chromosomes
Deoxyribonucleic acid
DNA
E coli
Escherichia coli
Escherichia coli - genetics
Gel electrophoresis
Gene mapping
Genetic testing
Genome, Bacterial - genetics
Genomes
Genomics
Health sciences
Laboratories
Mapping
Multidrug resistant organisms
Optics and Photonics - methods
Organisms
Shigella
Shigella - genetics
Surveillance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0027085