Phylogenetic diversity, host-specificity and community profiling of sponge-associated bacteria in the northern Gulf of Mexico

• Published in: PloS one 2011-11, Vol.6 (11), p.e26806-e26806
• Main Authors: Erwin, Patrick M, Olson, Julie B, Thacker, Robert W
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Bioinformatics; Biology; Chemical analysis; Communities; Community ecology; Comparative analysis; Consortia; Data analysis; Deoxyribonucleic acid; DNA; DNA fingerprinting; DNA identification; Evolution; Gene sequencing; Genetic fingerprinting; Genetic polymorphisms; Host specificity; Hymeniacidon heliophila; Integrated software; Microbial activity; Microorganisms; Phylogenetics; Phylogeny; Polymorphism, Restriction Fragment Length; Population genetics; Porifera - microbiology; Prey; Profiling; RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater; Seawater - microbiology; Software packages; Species composition; Species diversity; Species richness; Sponges; Studies; Symbionts; Symbiosis; Sympatric populations; Water analysis; Water Microbiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0026806

Marine sponges can associate with abundant and diverse consortia of microbial symbionts. However, associated bacteria remain unexamined for the majority of host sponges and few studies use phylogenetic metrics to quantify symbiont community diversity. DNA fingerprinting techniques, such as terminal restriction fragment length polymorphisms (T-RFLP), might provide rapid profiling of these communities, but have not been explicitly compared to traditional methods. We investigated the bacterial communities associated with the marine sponges Hymeniacidon heliophila and Haliclona tubifera, a sympatric tunicate, Didemnum sp., and ambient seawater from the northern Gulf of Mexico by combining replicated clone libraries with T-RFLP analyses of 16S rRNA gene sequences. Clone libraries revealed that bacterial communities associated with the two sponges exhibited lower species richness and lower species diversity than seawater and tunicate assemblages, with differences in species composition among all four source groups. T-RFLP profiles clustered microbial communities by source; individual T-RFs were matched to the majority (80.6%) of clone library sequences, indicating that T-RFLP analysis can be used to rapidly profile these communities. Phylogenetic metrics of community diversity indicated that the two sponge-associated bacterial communities include dominant and host-specific bacterial lineages that are distinct from bacteria recovered from seawater, tunicates, and unrelated sponge hosts. In addition, a large proportion of the symbionts associated with H. heliophila were shared with distant, conspecific host populations in the southwestern Atlantic (Brazil). The low diversity and species-specific nature of bacterial communities associated with H. heliophila and H. tubifera represent a distinctly different pattern from other, reportedly universal, sponge-associated bacterial communities. Our replicated sampling strategy, which included samples that reflect the ambient environment, allowed us to differentiate resident symbionts from potentially transient or prey bacteria. Pairing replicated clone library construction with rapid community profiling via T-RFLP analyses will greatly facilitate future studies of sponge-microbe symbioses.