Generation of micronuclei during interphase by coupling between cytoplasmic membrane blebbing and nuclear budding

• Published in: PloS one 2011-11, Vol.6 (11), p.e27233-e27233
• Main Authors: Utani, Koh-ichi, Okamoto, Atsushi, Shimizu, Noriaki
• Format: Article
• Language: English
• Subjects: Apoptosis; Biology; Biosphere; Blistering; Breaking; Budding; Camptothecin; Cancer; Cell cycle; Cell Line; Cell Membrane - metabolism; Cell membranes; Cell Nucleus - metabolism; Cell Nucleus - ultrastructure; Chromatin; Chromatin - metabolism; Chromosomes; Cytoplasm - metabolism; Cytoplasmic membranes; Cytoskeleton; Deoxyribonucleic acid; DNA; DNA methylation; Extrusion; Gene amplification; Gene expression; Genomes; Humans; Interphase; Lamin Type B - metabolism; Micronuclei; Microscopy; Nuclei; Plasmids; Proteins; S Phase; Tumor cell lines; Viral proteins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0027233

Micronucleation, mediated by interphase nuclear budding, has been repeatedly suggested, but the process is still enigmatic. In the present study, we confirmed the previous observation that there are lamin B1-negative micronuclei in addition to the positive ones. A large cytoplasmic bleb was found to frequently entrap lamin B1-negative micronuclei, which were connected to the nucleus by a thin chromatin stalk. At the bottom of the stalk, the nuclear lamin B1 structure appeared broken. Chromatin extrusion through lamina breaks has been referred to as herniation or a blister of the nucleus, and has been observed after the expression of viral proteins. A cell line in which extrachromosomal double minutes and lamin B1 protein were simultaneously visualized in different colors in live cells was established. By using these cells, time-lapse microscopy revealed that cytoplasmic membrane blebbing occurred simultaneously with the extrusion of nuclear content, which generated lamin B1-negative micronuclei during interphase. Furthermore, activation of cytoplasmic membrane blebbing by the addition of fresh serum or camptothecin induced nuclear budding within 1 to 10 minutes, which suggested that blebbing might be the cause of the budding. After the induction of blebbing, the frequency of lamin-negative micronuclei increased. The budding was most frequent during S phase and more efficiently entrapped small extrachromosomal chromatin than the large chromosome arm. Based on these results, we suggest a novel mechanism in which cytoplasmic membrane dynamics pulls the chromatin out of the nucleus through the lamina break. Evidence for such a mechanism was obtained in certain cancer cell lines including human COLO 320 and HeLa. The mechanism could significantly perturb the genome and influence cancer cell phenotypes.