Adult body weight is programmed by a redox-regulated and energy-dependent process during the pronuclear stage in mouse

In mammals fertilization triggers a series of Ca(2+) oscillations that not only are essential for events of egg activation but also stimulate oxidative phosphorylation. Little is known, however, about the relationship between quantitative changes in egg metabolism and specific long-term effects in o...

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Bibliographic Details
Published in:PloS one 2011-12, Vol.6 (12), p.e29388-e29388
Main Authors: Banrezes, Bernadette, Sainte-Beuve, Thierry, Canon, Eugénie, Schultz, Richard M, Cancela, José, Ozil, Jean-Pierre
Format: Article
Language:English
Subjects:
Alkalizing
Animals
Biology
Body Weight
Calcium signalling
Cell cycle
Development Biology
Eggs
Embryos
Energy Metabolism
Fertilization
Gene expression
Genomes
Growth rate
Lactic acid
Lactic Acid - pharmacology
Life Sciences
Long-term effects
Medicine
Metabolism
Mice
Mitochondria
Mitochondria - drug effects
Mitochondria - metabolism
NAD
NAD - metabolism
Offspring
Oscillations
Oxidation
Oxidation-Reduction
Oxidative Phosphorylation
Phosphorylation
Physiology
Progeny
Pyruvic acid
Redox potential
Weaning
Weight reduction
Zygotes
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Summary:In mammals fertilization triggers a series of Ca(2+) oscillations that not only are essential for events of egg activation but also stimulate oxidative phosphorylation. Little is known, however, about the relationship between quantitative changes in egg metabolism and specific long-term effects in offspring. This study assessed whether post-natal growth is modulated by early transient changes in NAD(P)H and FAD(2+) in zygotes. We report that experimentally manipulating the redox potential of fertilized eggs during the pronuclear (PN) stage affects post-natal body weight. Exogenous pyruvate induces NAD(P)H oxidation and stimulates mitochondrial activity with resulting offspring that are persistently and significantly smaller than controls. Exogenous lactate stimulates NAD(+) reduction and impairs mitochondrial activity, and produces offspring that are smaller than controls at weaning but catch up after weaning. Cytosolic alkalization increases NAD(P)(+) reduction and offspring of normal birth-weight become significantly and persistently larger than controls. These results constitute the first report that post-natal growth rate is ultimately linked to modulation of NAD(P)H and FAD(2+) concentration as early as the PN stage.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0029388