Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum

Microalgae are thought to offer great potential as expression system for various industrial, therapeutic and diagnostic recombinant proteins as they combine high growth rates with all benefits of eukaryotic expression systems. Moreover, microalgae exhibit a phototrophic lifestyle like land plants, h...

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Published in:PloS one 2011-12, Vol.6 (12), p.e28424-e28424
Main Authors: Hempel, Franziska, Lau, Julia, Klingl, Andreas, Maier, Uwe G
Format: Article
Language:English
Subjects:
Algae
Antibodies - chemistry
Antibodies, Monoclonal - chemistry
Antigens
Aquatic microorganisms
Bacillariophyta
Biochemistry - methods
Biodiesel fuels
Biology
Bioreactors
Carbon dioxide
Cell Line
Codon
Costs
Diagnostic systems
Diatoms - metabolism
E coli
Enzyme-Linked Immunosorbent Assay - methods
Genetic engineering
Green Fluorescent Proteins - metabolism
Growth rate
Health aspects
Hepatitis
Hepatitis B
Hepatitis B Surface Antigens - metabolism
Hepatitis B virus
Humans
IgG antibody
Immunoglobulin G
Immunoglobulin G - chemistry
Immunoglobulins
Industrial engineering
Industrial plants
Laboratories
Localization
Manufacturing engineering
Medicine
Microalgae - physiology
Microscopy, Fluorescence - methods
Morphogenesis
Nicotiana tabacum
Phaeodactylum tricornutum
Photosynthesis
Plankton
Plants (botany)
Plasmids - metabolism
Production costs
Protein Engineering - methods
Proteins
Recombinant proteins
Recombinant Proteins - chemistry
Tobacco
Transgenic plants
Vaccines
Viral infections
Yeast
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cited_by cdi_FETCH-LOGICAL-c757t-6c3dd653bba8a90bd19fa9a4b0885147b1d35c94d78e493437cf9f8539fe23683
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creator Hempel, Franziska
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description Microalgae are thought to offer great potential as expression system for various industrial, therapeutic and diagnostic recombinant proteins as they combine high growth rates with all benefits of eukaryotic expression systems. Moreover, microalgae exhibit a phototrophic lifestyle like land plants, hence protein expression is fuelled by photosynthesis, which is CO(2)-neutral and involves only low production costs. So far, however, research on algal bioreactors for recombinant protein expression is very rare calling for further investigations in this highly promising field. In this study, we present data on the expression of a monoclonal human IgG antibody against the Hepatitis B surface protein and the respective antigen in the diatom Phaeodactylum tricornutum. Antibodies are fully-assembled and functional and accumulate to 8.7% of total soluble protein, which complies with 21 mg antibody per gram algal dry weight. The Hepatitis B surface protein is functional as well and is recognized by algae-produced and commercial antibodies.
doi_str_mv 10.1371/journal.pone.0028424
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1932-6203
language eng
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subjects Algae
Antibodies - chemistry
Antibodies, Monoclonal - chemistry
Antigens
Aquatic microorganisms
Bacillariophyta
Biochemistry - methods
Biodiesel fuels
Biology
Bioreactors
Carbon dioxide
Cell Line
Codon
Costs
Diagnostic systems
Diatoms - metabolism
E coli
Enzyme-Linked Immunosorbent Assay - methods
Genetic engineering
Green Fluorescent Proteins - metabolism
Growth rate
Health aspects
Hepatitis
Hepatitis B
Hepatitis B Surface Antigens - metabolism
Hepatitis B virus
Humans
IgG antibody
Immunoglobulin G
Immunoglobulin G - chemistry
Immunoglobulins
Industrial engineering
Industrial plants
Laboratories
Localization
Manufacturing engineering
Medicine
Microalgae - physiology
Microscopy, Fluorescence - methods
Morphogenesis
Nicotiana tabacum
Phaeodactylum tricornutum
Photosynthesis
Plankton
Plants (botany)
Plasmids - metabolism
Production costs
Protein Engineering - methods
Proteins
Recombinant proteins
Recombinant Proteins - chemistry
Tobacco
Transgenic plants
Vaccines
Viral infections
Yeast
title Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum
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