Measurement of warfarin in the oral fluid of patients undergoing anticoagulant oral therapy

Patients on warfarin therapy undergo invasive and expensive checks for the coagulability of their blood. No information on coagulation levels is currently available between two controls. A method was developed to determine warfarin in oral fluid by HPLC and fluorimetric detection. The chromatographi...

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Bibliographic Details
Published in:PloS one 2011-12, Vol.6 (12), p.e28182-e28182
Main Authors: Ghimenti, Silvia, Lomonaco, Tommaso, Onor, Massimo, Murgia, Laura, Paolicchi, Aldo, Fuoco, Roger, Ruocco, Lucia, Pellegrini, Giovanni, Trivella, Maria Giovanna, Di Francesco, Fabio
Format: Article
Language:English
Subjects:
Administration, Oral
Adult
Aged
Aged, 80 and over
Anticoagulants
Anticoagulants - analysis
Anticoagulants - therapeutic use
Blood
Blood coagulation
Blood Coagulation - drug effects
Chemistry
Chromatography
Chromatography, High Pressure Liquid - methods
Coagulation
Coefficient of variation
Correlation
Correlation analysis
Cytochrome
Drug dosages
Drug Monitoring - methods
Embolisms
Female
Flow velocity
Fluids
Fluorometry - methods
High performance liquid chromatography
Humans
Hydrogen ions
Hydrogen-Ion Concentration
International Normalized Ratio
Laboratories
Liquid chromatography
Male
Mass spectrometry
Mathematical analysis
Matrix methods
Measurement
Medicine
Metabolism
Metabolites
Methanol
Middle Aged
Patients
pH effects
Plasma
Potassium
Principal Component Analysis
Principal components analysis
Proteins
Reproducibility of Results
Saliva - drug effects
Saliva - metabolism
Scientific imaging
Therapy
Values
Warfarin
Warfarin - analysis
Warfarin - therapeutic use
Wavelength
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Description
Summary:Patients on warfarin therapy undergo invasive and expensive checks for the coagulability of their blood. No information on coagulation levels is currently available between two controls. A method was developed to determine warfarin in oral fluid by HPLC and fluorimetric detection. The chromatographic separation was performed at room temperature on a C-18 reversed-phase column, 65% PBS and 35% methanol mobile phase, flow rate 0.7 mL/min, injection volume 25 µL, excitation wavelength 310 nm, emission wavelength 400 nm. The method was free from interference and matrix effect, linear in the range 0.2-100 ng/mL, with a detection limit of 0.2 ng/mL. Its coefficient of variation was
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0028182