Galectin-1 is part of human trophoblast invasion machinery--a functional study in vitro

• Published in: PloS one 2011-12, Vol.6 (12), p.e28514
• Main Authors: Kolundžić, Nikola, Bojić-Trbojević, Žanka, Kovačević, Tamara, Stefanoska, Ivana, Kadoya, Toshihiko, Vićovac, Ljiljana
• Format: Article
• Language: English
• Subjects: Adhesion tests; Antibodies; Antibodies, Blocking - pharmacology; Apoptosis; Biology; Breweries; Cell adhesion; Cell adhesion & migration; Cell Adhesion - drug effects; Cell Line; Cell Movement - drug effects; Cell Separation; Cell Survival - drug effects; Colorectal cancer; Cytokines; Extracellular matrix; Flow Cytometry; Galectin 1 - immunology; Galectin 1 - metabolism; Galectin 3 - metabolism; Galectin-1; Galectins - metabolism; Glycoconjugates; Humans; Hypoxia; Implantation; Laboratories; Lactose - pharmacology; Lectins; Liver cancer; Localization; Machinery; Machinery and equipment; Medicine; Pancreatic cancer; Phosphorylation; Placenta; Polymerase chain reaction; Pregnancy; Protein Binding - drug effects; Proteins; Recombinant Proteins - metabolism; Trophoblasts - cytology; Trophoblasts - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0028514

Interactions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it. Function blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM ± 6.3, P