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Immature cryopreserved ovary restores puberty and fertility in mice without alteration of epigenetic marks
Progress in oncology could improve survival rate in children, but would probably lead to impaired fertility and puberty. In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conver...
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| Published in: | PloS one 2008-04, Vol.3 (4), p.e1972-e1972 |
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| creator | Sauvat, Frédérique Capito, Carmen Sarnacki, Sabine Poirot, Catherine Bachelot, Anne Meduri, Geri Dandolo, Luisa Binart, Nadine |
| description | Progress in oncology could improve survival rate in children, but would probably lead to impaired fertility and puberty. In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conversely, reimplantation of ovary preserved before puberty (defined as immature ovary) has never been performed in humans.
In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice.
These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women. |
| doi_str_mv | 10.1371/journal.pone.0001972 |
| format | article |
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In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice.
These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0001972</identifier><identifier>PMID: 18414667</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biopsy ; Births ; Cancer ; Children ; Chromosomes ; Cryopreservation ; Cryopreservation - methods ; Diabetes and Endocrinology/Reproductive Endocrinology ; DNA methylation ; Drug dosages ; Epigenesis, Genetic ; Epigenetic inheritance ; Epigenetics ; Female ; Fertility ; Genes ; Genetic engineering ; Genetics and Genomics/Epigenetics ; Genomic imprinting ; Girls ; Grafting ; Grafts ; Health aspects ; Imprinting ; Infertility ; Laparoscopy ; Life span ; Mammary Glands, Animal - pathology ; Medicine ; Methylation ; Mice ; Organ transplantation ; Ovarian Follicle - pathology ; Ovary - pathology ; Ovary - physiology ; Ovary - transplantation ; Pediatrics ; Pediatrics and Child Health/Pediatric Oncology ; Physiology/Endocrinology ; Pregnancy ; Puberty ; Reproductive status ; Reproductive Techniques ; Reproductive technologies ; Sexual Maturation ; Survival ; Time Factors ; Tongue ; Transplantation ; Transplants & implants ; Uterus - pathology</subject><ispartof>PloS one, 2008-04, Vol.3 (4), p.e1972-e1972</ispartof><rights>COPYRIGHT 2008 Public Library of Science</rights><rights>2008 Sauvat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Sauvat et al. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c662t-2974139114b46fe319408ce8d3686fde02b41ae0443271c96d56234062292fab3</citedby><cites>FETCH-LOGICAL-c662t-2974139114b46fe319408ce8d3686fde02b41ae0443271c96d56234062292fab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1312203064/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1312203064?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18414667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kim, Samuel</contributor><creatorcontrib>Sauvat, Frédérique</creatorcontrib><creatorcontrib>Capito, Carmen</creatorcontrib><creatorcontrib>Sarnacki, Sabine</creatorcontrib><creatorcontrib>Poirot, Catherine</creatorcontrib><creatorcontrib>Bachelot, Anne</creatorcontrib><creatorcontrib>Meduri, Geri</creatorcontrib><creatorcontrib>Dandolo, Luisa</creatorcontrib><creatorcontrib>Binart, Nadine</creatorcontrib><title>Immature cryopreserved ovary restores puberty and fertility in mice without alteration of epigenetic marks</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Progress in oncology could improve survival rate in children, but would probably lead to impaired fertility and puberty. In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conversely, reimplantation of ovary preserved before puberty (defined as immature ovary) has never been performed in humans.
In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice.
These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biopsy</subject><subject>Births</subject><subject>Cancer</subject><subject>Children</subject><subject>Chromosomes</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Diabetes and Endocrinology/Reproductive Endocrinology</subject><subject>DNA methylation</subject><subject>Drug dosages</subject><subject>Epigenesis, Genetic</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Female</subject><subject>Fertility</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetics and Genomics/Epigenetics</subject><subject>Genomic imprinting</subject><subject>Girls</subject><subject>Grafting</subject><subject>Grafts</subject><subject>Health aspects</subject><subject>Imprinting</subject><subject>Infertility</subject><subject>Laparoscopy</subject><subject>Life span</subject><subject>Mammary Glands, Animal - 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In pre-pubertal girls, the only therapeutic option is the cryopreservation of one ovary. Three births have been reported after reimplantation of cryopreserved mature ovary. Conversely, reimplantation of ovary preserved before puberty (defined as immature ovary) has never been performed in humans.
In order to analyze ovarian function, we performed transplantation using fresh or cryopreserved immature grafts in pre-pubertal or adult mice. Puberty as well as cyclic hormonal activity was restored. All follicle populations were present although a significant reduction in follicle density was observed with or without cryopreservation. Although fertility was restored, the graft is of limited life span. Because ex vivo ovary manipulation and cryopreservation procedure, the status of genomic imprinting was investigated. Methylation status of the H19 and Lit1 Imprinting Control Regions in kidney, muscle and tongue of offsprings from grafted mice does not show significant alteration when compared to those of unoperated mice.
These results demonstrate that immature ovarian grafting can restore spontaneous puberty and fertility. However, these data suggest that follicle depletion leads to premature ovarian failure. This study addresses the very important epigenetics issue, and provides valuable information to the study of ovarian transplantation suggesting that these procedures do not perturb normal epigenetics marks. These results are highly relevant to the reimplantation question of immature cortex in women.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>18414667</pmid><doi>10.1371/journal.pone.0001972</doi><tpages>e1972</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2008-04, Vol.3 (4), p.e1972-e1972 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1312203064 |
| source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central |
| subjects | Analysis Animals Biopsy Births Cancer Children Chromosomes Cryopreservation Cryopreservation - methods Diabetes and Endocrinology/Reproductive Endocrinology DNA methylation Drug dosages Epigenesis, Genetic Epigenetic inheritance Epigenetics Female Fertility Genes Genetic engineering Genetics and Genomics/Epigenetics Genomic imprinting Girls Grafting Grafts Health aspects Imprinting Infertility Laparoscopy Life span Mammary Glands, Animal - pathology Medicine Methylation Mice Organ transplantation Ovarian Follicle - pathology Ovary - pathology Ovary - physiology Ovary - transplantation Pediatrics Pediatrics and Child Health/Pediatric Oncology Physiology/Endocrinology Pregnancy Puberty Reproductive status Reproductive Techniques Reproductive technologies Sexual Maturation Survival Time Factors Tongue Transplantation Transplants & implants Uterus - pathology |
| title | Immature cryopreserved ovary restores puberty and fertility in mice without alteration of epigenetic marks |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T16%3A08%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immature%20cryopreserved%20ovary%20restores%20puberty%20and%20fertility%20in%20mice%20without%20alteration%20of%20epigenetic%20marks&rft.jtitle=PloS%20one&rft.au=Sauvat,%20Fr%C3%A9d%C3%A9rique&rft.date=2008-04-16&rft.volume=3&rft.issue=4&rft.spage=e1972&rft.epage=e1972&rft.pages=e1972-e1972&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0001972&rft_dat=%3Cgale_plos_%3EA472653359%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c662t-2974139114b46fe319408ce8d3686fde02b41ae0443271c96d56234062292fab3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1312203064&rft_id=info:pmid/18414667&rft_galeid=A472653359&rfr_iscdi=true |