The CD81 partner EWI-2wint inhibits hepatitis C virus entry

Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry fac...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2008-04, Vol.3 (4), p.e1866-e1866
Main Authors: Rocha-Perugini, Vera, Montpellier, Claire, Delgrange, David, Wychowski, Czeslaw, Helle, François, Pillez, André, Drobecq, Hervé, Le Naour, François, Charrin, Stéphanie, Levy, Shoshana, Rubinstein, Eric, Dubuisson, Jean, Cocquerel, Laurence
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, CD - chemistry
Antigens, CD - physiology
CD81 antigen
Cell culture
Cell Line, Tumor
Cell Membrane - metabolism
Cell surface
Cercopithecus aethiops
CHO Cells
COS Cells
Cricetinae
Cricetulus
Development and progression
Disease susceptibility
Ectopic expression
Endoplasmic reticulum
Glycoproteins
Glycoproteins - chemistry
Health aspects
Health risks
Hepacivirus - metabolism
Hepatitis
Hepatitis C
Hepatocytes
Hepatocytes - metabolism
Hepatoma
Humans
Infection
Liver
Liver cancer
Localization
Membrane Proteins - chemistry
Membrane Proteins - physiology
Molecular Sequence Data
Motility
Mutation
Proteins
Sequence Homology, Amino Acid
Tetraspanin 28
U937 Cells
Virology/Host Invasion and Cell Entry
Virology/Mechanisms of Resistance and Susceptibility, including Host Genetics
Virology/Viruses and Cancer
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two to three percent of the world's population is chronically infected with hepatitis C virus (HCV) and thus at risk of developing liver cancer. Although precise mechanisms regulating HCV entry into hepatic cells are still unknown, several cell surface proteins have been identified as entry factors for this virus. Among these molecules, the tetraspanin CD81 is essential for HCV entry. Here, we have identified a partner of CD81, EWI-2wint, which is expressed in several cell lines but not in hepatocytes. Ectopic expression of EWI-2wint in a hepatoma cell line susceptible to HCV infection blocked viral entry by inhibiting the interaction between the HCV envelope glycoproteins and CD81. This finding suggests that, in addition to the presence of specific entry factors in the hepatocytes, the lack of a specific inhibitor can contribute to the hepatotropism of HCV. This is the first example of a pathogen gaining entry into host cells that lack a specific inhibitory factor.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0001866