Nod2 mediates susceptibility to Yersinia pseudotuberculosis in mice

• Published in: PloS one 2008-07, Vol.3 (7), p.e2769-e2769
• Main Authors: Meinzer, Ulrich, Esmiol-Welterlin, Sophie, Barreau, Frederick, Berrebi, Dominique, Dussaillant, Monique, Bonacorsi, Stephane, Chareyre, Fabrice, Niwa-Kawakita, Michiko, Alberti, Corinne, Sterkers, Ghislaine, Villard, Claude, Lesuffleur, Thecla, Peuchmaur, Michel, Karin, Michael, Eckmann, Lars, Giovannini, Marco, Ollendorff, Vincent, Wolf-Watz, Hans, Hugot, Jean-Pierre
• Format: Article
• Language: English
• Subjects: Age; Animals; Antigens; Apoptosis; Bacteria; Bone Marrow Cells - metabolism; Cellular Biology; Cloning; Crohn's disease; Crohns disease; Diderot, Denis (1713-1784); Disease Susceptibility; Drug dosages; Gastroenteritis; Gastroenterology; Gastroenterology and Hepatology/Inflammatory Bowel Disease; Gene Deletion; Gene expression; Genetic Predisposition to Disease; Health aspects; Homeostasis; Immune response; Immune system; Immunity; Infection; Infections; Infectious Diseases/Gastrointestinal Infections; Infiltration; Inflammatory bowel disease; Innate immunity; Kinases; Leukocytes (neutrophilic); Life Sciences; Lymphatic system; Macrophages; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microbiology/Immunity to Infections; Microbiology/Innate Immunity; Molecular biology; Mycobacterium bovis; Mycobacterium tuberculosis; NOD2 protein; Nod2 Signaling Adaptor Protein - genetics; Nod2 Signaling Adaptor Protein - physiology; Organs; Peyer's Patches - microbiology; Phenotype; Pseudotuberculosis; Rodents; Signal transduction; Tuberculosis; Vaccines; Yersinia; Yersinia enterocolitica; Yersinia pseudotuberculosis; Yersinia pseudotuberculosis - genetics; Yersinia pseudotuberculosis Infections - genetics; Yersinia pseudotuberculosis Infections - microbiology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0002769

Nucleotide oligomerisation domain 2 (NOD2) is a component of the innate immunity known to be involved in the homeostasis of Peyer patches (PPs) in mice. However, little is known about its role during gut infection in vivo. Yersinia pseudotuberculosis is an enteropathogen causing gastroenteritis, adenolymphitis and septicaemia which is able to invade its host through PPs. We investigated the role of Nod2 during Y. pseudotuberculosis infection. Death was delayed in Nod2 deleted and Crohn's disease associated Nod2 mutated mice orogastrically inoculated with Y. pseudotuberculosis. In PPs, the local immune response was characterized by a higher KC level and a more intense infiltration by neutrophils and macrophages. The apoptotic and bacterial cell counts were decreased. Finally, Nod2 deleted mice had a lower systemic bacterial dissemination and less damage of the haematopoeitic organs. This resistance phenotype was lost in case of intraperitoneal infection. We concluded that Nod2 contributes to the susceptibility to Y. pseudotuberculosis in mice.