Reduced basal ATP synthetic flux of skeletal muscle in patients with previous acromegaly

• Published in: PloS one 2008-12, Vol.3 (12), p.e3958-e3958
• Main Authors: Szendroedi, Julia, Zwettler, Elisabeth, Schmid, Albrecht Ingo, Chmelik, Marek, Pacini, Giovanni, Kacerovsky, Gertrud, Smekal, Gerhard, Nowotny, Peter, Wagner, Oswald, Schnack, Christoph, Schernthaner, Guntram, Klaushofer, Klaus, Roden, Michael
• Format: Article
• Language: English
• Subjects: Acromegaly; Acromegaly - metabolism; Acromegaly - physiopathology; Acromegaly - rehabilitation; Adenosine Triphosphate - metabolism; Adult; ATP; Basal Metabolism - physiology; Biological Transport - physiology; Body mass; Body mass index; Body size; Boltzmann, Ludwig Eduard (1844-1906); Calorimetry; Care and treatment; Case-Control Studies; Diabetes; Diabetes and Endocrinology/Endocrinology; Disease; Down-Regulation; Endocrinology; Exercise; Exercise - physiology; Fatty acids; Female; Glucose; Glucose metabolism; Glucose Tolerance Test; Growth hormone; Growth hormones; Hospitals; Humans; Insulin; Insulin resistance; Insulin Resistance - physiology; Insulin secretion; Insulin-like growth factor I; Insulin-like growth factors; Lipid metabolism; Lipid Metabolism - physiology; Lipids; Liver; Magnetic resonance; Magnetic resonance spectroscopy; Male; Medicine; Metabolism; Metabolites; Middle Aged; Mitochondria; Mitochondria, Muscle - metabolism; Mortality; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Nuclear magnetic resonance spectroscopy; Older people; Oxygen consumption; Oxygen Consumption - physiology; Pathogenesis; Patients; Phosphorylation; Physical fitness; Physiological aspects; Physiology/Endocrinology; Physiology/Muscle and Connective Tissue; Plasma; Rodents; Secretion; Sensitivity; Sensitivity analysis; Skeletal muscle; Somatotropin; Spectroscopy; Spectrum analysis; Studies; Tumors; Type 2 diabetes
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0003958

Impaired mitochondrial function and ectopic lipid deposition in skeletal muscle and liver have been linked to decreased insulin sensitivity. As growth hormone (GH) excess can reduce insulin sensitivity, we examined the impact of previous acromegaly (AM) on glucose metabolism, lipid storage and muscular ATP turnover. Seven AM (4f/3 m, age: 46+/-4 years, BMI: 28+/-1 kg/m(2)) and healthy volunteers (CON: 3f/4 m, 43+/-4 years, 26+/-2 kg/m(2)) matched for age and body mass underwent oral glucose testing for assessment of insulin sensitivity (OGIS) and ss-cell function (adaptation index, ADAP). Whole body oxidative capacity was measured with indirect calorimetry and spiroergometry. Unidirectional ATP synthetic flux (fATP) was assessed from (31)P magnetic resonance spectroscopy (MRS) of calf muscle. Lipid contents of tibialis anterior (IMCLt) and soleus muscles (IMCLs) and liver (HCL) were measured with (1)H MRS. Despite comparable GH, insulin-like growth factor-1 (IGF-I) and insulin sensitivity, AM had approximately 85% lower ADAP (p