Sequential assembly of centromeric proteins in male mouse meiosis

The assembly of the mitotic centromere has been extensively studied in recent years, revealing the sequence and regulation of protein loading to this chromosome domain. However, few studies have analyzed centromere assembly during mammalian meiosis. This study specifically targets this approach on m...

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Bibliographic Details
Published in:PLoS genetics 2009-03, Vol.5 (3), p.e1000417-e1000417
Main Authors: Parra, María Teresa, Gómez, Rocío, Viera, Alberto, Llano, Elena, Pendás, Alberto M, Rufas, Julio S, Suja, José A
Format: Article
Language:English
Subjects:
Animals
Cell Biology
Cell cycle
Cell Cycle Proteins - metabolism
Cell division
Cellular biology
Centromere - chemistry
Centromere - metabolism
Chromosomal proteins
Chromosomal Proteins, Non-Histone - metabolism
Genetic aspects
Genetics and Genomics/Chromosome Biology
Genetics and Genomics/Nuclear Structure and Function
Kinesin - metabolism
Kinetochores - chemistry
Kinetochores - metabolism
Male
Meiosis
Meiotic Prophase I
Mice
Mitosis
Physiological aspects
Protein-Serine-Threonine Kinases - metabolism
Proteins
Spermatocytes - ultrastructure
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Summary:The assembly of the mitotic centromere has been extensively studied in recent years, revealing the sequence and regulation of protein loading to this chromosome domain. However, few studies have analyzed centromere assembly during mammalian meiosis. This study specifically targets this approach on mouse spermatocytes. We have found that during prophase I, the proteins of the chromosomal passenger complex Borealin, INCENP, and Aurora-B load sequentially to the inner centromere before Shugoshin 2 and MCAK. The last proteins to be assembled are the outer kinetochore proteins BubR1 and CENP-E. All these proteins are not detected at the centromere during anaphase/telophase I and are then reloaded during interkinesis. The loading sequence of the analyzed proteins is similar during prophase I and interkinesis. These findings demonstrate that the interkinesis stage, regularly overlooked, is essential for centromere and kinetochore maturation and reorganization previous to the second meiotic division. We also demonstrate that Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1000417