DNA methyltransferases are required to induce heterochromatic re-replication in Arabidopsis

The relationship between epigenetic marks on chromatin and the regulation of DNA replication is poorly understood. Mutations of the H3K27 methyltransferase genes, Arabidopsis trithorax-related protein5 (ATXR5) and ATXR6, result in re-replication (repeated origin firing within the same cell cycle). H...

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Bibliographic Details
Published in:PLoS genetics 2012-07, Vol.8 (7), p.e1002808-e1002808
Main Authors: Stroud, Hume, Hale, Christopher J, Feng, Suhua, Caro, Elena, Jacob, Yannick, Michaels, Scott D, Jacobsen, Steven E
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Biology
Cell Cycle - genetics
Deoxyribonucleic acid
DNA
DNA methylation
DNA Methylation - genetics
DNA replication
DNA Replication - genetics
DNA Transposable Elements - genetics
Epigenesis, Genetic - genetics
Gene Expression
Genetic aspects
Genetics
Health aspects
Heterochromatin - genetics
Histone-Lysine N-Methyltransferase - genetics
Homologous Recombination
Methyltransferases
Methyltransferases - genetics
Methyltransferases - metabolism
Mutation
Physiological aspects
Plant genetics
Proteins
Replication Origin - genetics
Sequence Analysis, RNA
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Summary:The relationship between epigenetic marks on chromatin and the regulation of DNA replication is poorly understood. Mutations of the H3K27 methyltransferase genes, Arabidopsis trithorax-related protein5 (ATXR5) and ATXR6, result in re-replication (repeated origin firing within the same cell cycle). Here we show that mutations that reduce DNA methylation act to suppress the re-replication phenotype of atxr5 atxr6 mutants. This suggests that DNA methylation, a mark enriched at the same heterochromatic regions that re-replicate in atxr5/6 mutants, is required for aberrant re-replication. In contrast, RNA sequencing analyses suggest that ATXR5/6 and DNA methylation cooperatively transcriptionally silence transposable elements (TEs). Hence our results suggest a complex relationship between ATXR5/6 and DNA methylation in the regulation of DNA replication and transcription of TEs.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1002808