Poly(ADP-Ribose) polymerase 1 (PARP-1) regulates ribosomal biogenesis in Drosophila nucleoli

• Published in: PLoS genetics 2012-01, Vol.8 (1), p.e1002442-e1002442
• Main Authors: Boamah, Ernest K, Kotova, Elena, Garabedian, Mikael, Jarnik, Michael, Tulin, Alexei V
• Format: Article
• Language: English
• Subjects: Animals; Animals, Genetically Modified; Biology; Biosynthesis; Cell growth; Cell Nucleolus - enzymology; Cell Nucleolus - ultrastructure; Cytoplasm; Deoxyribonucleic acid; DNA; DNA polymerases; Drosophila; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Gene Expression Regulation; Genetic aspects; In Situ Hybridization, Fluorescence; Insects; Kinases; Mutation; Nuclear Proteins - metabolism; Physiological aspects; Poly (ADP-Ribose) Polymerase-1; Poly Adenosine Diphosphate Ribose - metabolism; Poly(ADP-ribose) Polymerases - genetics; Poly(ADP-ribose) Polymerases - metabolism; Proteins; Ribosomal RNA; Ribosomes - genetics; Ribosomes - metabolism; Ribosomes - ultrastructure; RNA Interference; RNA Precursors - genetics; RNA Precursors - metabolism; RNA, Ribosomal - genetics; RNA, Ribosomal - metabolism; Structure
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1002442

Poly(ADP-ribose) polymerase 1 (PARP1), a nuclear protein, utilizes NAD to synthesize poly(AD-Pribose) (pADPr), resulting in both automodification and the modification of acceptor proteins. Substantial amounts of PARP1 and pADPr (up to 50%) are localized to the nucleolus, a subnuclear organelle known as a region for ribosome biogenesis and maturation. At present, the functional significance of PARP1 protein inside the nucleolus remains unclear. Using PARP1 mutants, we investigated the function of PARP1, pADPr, and PARP1-interacting proteins in the maintenance of nucleolus structure and functions. Our analysis shows that disruption of PARP1 enzymatic activity caused nucleolar disintegration and aberrant localization of nucleolar-specific proteins. Additionally, PARP1 mutants have increased accumulation of rRNA intermediates and a decrease in ribosome levels. Together, our data suggests that PARP1 enzymatic activity is required for targeting nucleolar proteins to the proximity of precursor rRNA; hence, PARP1 controls precursor rRNA processing, post-transcriptional modification, and pre-ribosome assembly. Based on these findings, we propose a model that explains how PARP1 activity impacts nucleolar functions and, consequently, ribosomal biogenesis.