Variations in Helicobacter pylori cytotoxin-associated genes and their influence in progression to gastric cancer: implications for prevention

Helicobacter pylori (HP) is a bacterium that colonizes the human stomach and can establish a long-term infection of the gastric mucosa. Persistent Hp infection often induces gastritis and is associated with the development of peptic ulcer disease, atrophic gastritis, and gastric adenocarcinoma. Viru...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-01, Vol.7 (1), p.e29605-e29605
Main Authors: Rizzato, Cosmeri, Torres, Javier, Plummer, Martyn, Muñoz, Nubia, Franceschi, Silvia, Camorlinga-Ponce, Margarita, Fuentes-Pananá, Ezequiel M, Canzian, Federico, Kato, Ikuko
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adult
Aged
Amino acids
Analysis
Antibiotics
Bacterial infections
Bias
Biology
Biopsy
Cages
Cancer
Cancer genetics
Chronic illnesses
Clinical isolates
Clinical outcomes
Codons
Deoxyribonucleic acid
Development and progression
Disease Progression
DNA
E coli
Escherichia coli
Ethics
Female
Gastric cancer
Gastric mucosa
Gastritis
Gastroenterology
Gene expression
Genes
Genes, Viral - genetics
Genetic diversity
Genetic testing
Genetic variability
Genetic variance
Genetic Variation
Genomes
Genomic Islands - genetics
Health aspects
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - physiology
Homology
Humans
Hypotheses
Infection
Infections
Kinases
Male
Medical research
Medicine
Middle Aged
Pathogenicity
Pathogens
Peptic ulcer
Risk
Secretion
Single-nucleotide polymorphism
Statistical analysis
Statistical methods
Stomach
Stomach cancer
Stomach Neoplasms - microbiology
Stomach Neoplasms - pathology
Stomach Neoplasms - prevention & control
Trinucleotide repeats
Virulence
Virulence (Microbiology)
Virulence factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Helicobacter pylori (HP) is a bacterium that colonizes the human stomach and can establish a long-term infection of the gastric mucosa. Persistent Hp infection often induces gastritis and is associated with the development of peptic ulcer disease, atrophic gastritis, and gastric adenocarcinoma. Virulent HP isolates harbor the cag (cytotoxin-associated genes) pathogenicity island (cagPAI), a 40 kb stretch of DNA that encodes components of a type IV secretion system (T4SS). This T4SS forms a pilus for the injection of virulence factors into host target cells, such as the CagA oncoprotein. We analyzed the genetic variability in cagA and other selected genes of the HP cagPAI (cagC, cagE, cagL, cagT, cagV and cag Gamma) using DNA extracted from frozen gastric biopsies or from clinical isolates. Study subjects were 95 cagA+ patients that were histologically diagnosed with chronic gastritis or gastric cancer in Venezuela and Mexico, areas with high prevalence of Hp infection. Sequencing reactions were carried out by both Sanger and next-generation pyrosequencing (454 Roche) methods. We found a total of 381 variants with unambiguous calls observed in at least 10% of the originally tested samples and reference strains. We compared the frequencies of these genetic variants between gastric cancer and chronic gastritis cases. Twenty-six SNPs (11 non-synonymous and 14 synonymous) showed statistically significant differences (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0029605