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Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols
Polyphenols are the most abundant antioxidants in the human diet and are widespread constituents of fruits and beverages, such as tea, coffee or wine. Epidemiological, clinical and animal studies support a role of polyphenols in the prevention of various diseases, such as cardiovascular diseases, ca...
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Published in: | PloS one 2012-01, Vol.7 (1), p.e29837-167 |
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description | Polyphenols are the most abundant antioxidants in the human diet and are widespread constituents of fruits and beverages, such as tea, coffee or wine. Epidemiological, clinical and animal studies support a role of polyphenols in the prevention of various diseases, such as cardiovascular diseases, cancers or neurodegenerative diseases. Recent findings suggest that polyphenols could interact with cellular signaling cascades regulating the activity of transcription factors and consequently affecting the expression of genes. However, the impact of polyphenol on the expression of microRNA, small non-coding RNAs, has not yet been studied. The aim of this study was to investigate the impact of dietary supplementation with polyphenols at nutritional doses on miRNA expression in the livers of apolipoprotein E-deficient mice (apoE⁻/⁻) jointly with mRNA expression profiling.
Using microarrays, we measured the global miRNA expression in the livers of wild-type (C57B6/J) mice or apoE⁻/⁻ mice fed diets supplemented with one of nine different polyphenols or a control diet. This analysis revealed that knock-out of the apoE gene induced significant modulation in the expression of miRNA. Moreover, changes in miRNA expression were observed after polyphenol supplementation, and five miRNAs (mmu-miR-291b-5p, mmu-miR-296-5p, mmu-miR-30c-1*, mmu-miR-467b* and mmu-miR-374*) were identified as being commonly modulated by these polyphenols. We also observed that these polyphenols counteracted the modulation of miRNA expression induced by apoE mutation. Pathway analyses on these five miRNA-target genes revealed common pathways, some of which were also identified from a pathway analysis on mRNA profiles.
This in vivo study demonstrated for the first time that polyphenols at nutritional doses modulate the expression of miRNA in the liver. Even if structurally different, all polyphenols induced a similar miRNA expression profile. Common pathways were identified from both miRNA-target and mRNA analysis, revealing cellular functions that could be regulated by polyphenols at both the miRNA and mRNA level. |
doi_str_mv | 10.1371/journal.pone.0029837 |
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Using microarrays, we measured the global miRNA expression in the livers of wild-type (C57B6/J) mice or apoE⁻/⁻ mice fed diets supplemented with one of nine different polyphenols or a control diet. This analysis revealed that knock-out of the apoE gene induced significant modulation in the expression of miRNA. Moreover, changes in miRNA expression were observed after polyphenol supplementation, and five miRNAs (mmu-miR-291b-5p, mmu-miR-296-5p, mmu-miR-30c-1*, mmu-miR-467b* and mmu-miR-374*) were identified as being commonly modulated by these polyphenols. We also observed that these polyphenols counteracted the modulation of miRNA expression induced by apoE mutation. Pathway analyses on these five miRNA-target genes revealed common pathways, some of which were also identified from a pathway analysis on mRNA profiles.
This in vivo study demonstrated for the first time that polyphenols at nutritional doses modulate the expression of miRNA in the liver. Even if structurally different, all polyphenols induced a similar miRNA expression profile. Common pathways were identified from both miRNA-target and mRNA analysis, revealing cellular functions that could be regulated by polyphenols at both the miRNA and mRNA level.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0029837</identifier><identifier>PMID: 22253797</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin Cytoskeleton - drug effects ; Actin Cytoskeleton - genetics ; Analysis ; Animals ; Antioxidants ; Antioxidants (Nutrients) ; Apolipoprotein E ; Apolipoproteins ; Apolipoproteins E - deficiency ; Apolipoproteins E - metabolism ; Apoptosis ; Arabidopsis thaliana ; Atherosclerosis ; Beverages ; Biology ; Cancer ; Cardiovascular diseases ; Cascades ; Cell cycle ; Citrus paradisi ; Cluster Analysis ; Coding ; Coffee ; Coronary vessels ; Diet ; Dietary supplements ; Diseases ; DNA binding proteins ; DNA microarrays ; Down-Regulation - drug effects ; Down-Regulation - genetics ; Epidemiology ; Fatty acids ; Flavonoids ; Food ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation - drug effects ; Genes ; Genetic research ; Heart diseases ; Humans ; In vivo methods and tests ; Laboratories ; Life Sciences ; Liver ; Liver - drug effects ; Liver - metabolism ; Medical research ; Medicine ; Metabolites ; Mice ; Mice, Inbred C57BL ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Modulation ; Mutation ; Nervous system diseases ; Neurodegenerative diseases ; Neurological diseases ; Neutrophils ; Non-coding RNA ; Nutrition ; Oligonucleotide Array Sequence Analysis ; Oxidative stress ; Pathogenesis ; Pathways ; Polyphenols ; Polyphenols - pharmacology ; Proteins ; Ribonucleic acid ; RNA ; RNA polymerase ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signaling ; Supplementation ; Target recognition ; Tea ; Transcription (Genetics) ; Transcription factors ; Up-Regulation - drug effects ; Up-Regulation - genetics ; Wine</subject><ispartof>PloS one, 2012-01, Vol.7 (1), p.e29837-167</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Milenkovic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Milenkovic et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c791t-52c17b8d43396def26594737b04ca3db6c39e344e65b6843c446681cd94dfcb23</citedby><cites>FETCH-LOGICAL-c791t-52c17b8d43396def26594737b04ca3db6c39e344e65b6843c446681cd94dfcb23</cites><orcidid>0000-0002-1067-5066 ; 0000-0001-6353-0912 ; 0000-0001-6651-6710 ; 0000-0001-8128-1032 ; 0000-0002-8690-8014</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1322478378/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1322478378?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22253797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02650784$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Krahe, Ralf</contributor><creatorcontrib>Milenkovic, Dragan</creatorcontrib><creatorcontrib>Deval, Christiane</creatorcontrib><creatorcontrib>Gouranton, Erwan</creatorcontrib><creatorcontrib>Landrier, Jean-François</creatorcontrib><creatorcontrib>Scalbert, Augustin</creatorcontrib><creatorcontrib>Morand, Christine</creatorcontrib><creatorcontrib>Mazur, Andrzej</creatorcontrib><title>Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Polyphenols are the most abundant antioxidants in the human diet and are widespread constituents of fruits and beverages, such as tea, coffee or wine. Epidemiological, clinical and animal studies support a role of polyphenols in the prevention of various diseases, such as cardiovascular diseases, cancers or neurodegenerative diseases. Recent findings suggest that polyphenols could interact with cellular signaling cascades regulating the activity of transcription factors and consequently affecting the expression of genes. However, the impact of polyphenol on the expression of microRNA, small non-coding RNAs, has not yet been studied. The aim of this study was to investigate the impact of dietary supplementation with polyphenols at nutritional doses on miRNA expression in the livers of apolipoprotein E-deficient mice (apoE⁻/⁻) jointly with mRNA expression profiling.
Using microarrays, we measured the global miRNA expression in the livers of wild-type (C57B6/J) mice or apoE⁻/⁻ mice fed diets supplemented with one of nine different polyphenols or a control diet. This analysis revealed that knock-out of the apoE gene induced significant modulation in the expression of miRNA. Moreover, changes in miRNA expression were observed after polyphenol supplementation, and five miRNAs (mmu-miR-291b-5p, mmu-miR-296-5p, mmu-miR-30c-1*, mmu-miR-467b* and mmu-miR-374*) were identified as being commonly modulated by these polyphenols. We also observed that these polyphenols counteracted the modulation of miRNA expression induced by apoE mutation. Pathway analyses on these five miRNA-target genes revealed common pathways, some of which were also identified from a pathway analysis on mRNA profiles.
This in vivo study demonstrated for the first time that polyphenols at nutritional doses modulate the expression of miRNA in the liver. Even if structurally different, all polyphenols induced a similar miRNA expression profile. Common pathways were identified from both miRNA-target and mRNA analysis, revealing cellular functions that could be regulated by polyphenols at both the miRNA and mRNA level.</description><subject>Actin Cytoskeleton - drug effects</subject><subject>Actin Cytoskeleton - genetics</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Apolipoprotein E</subject><subject>Apolipoproteins</subject><subject>Apolipoproteins E - deficiency</subject><subject>Apolipoproteins E - metabolism</subject><subject>Apoptosis</subject><subject>Arabidopsis thaliana</subject><subject>Atherosclerosis</subject><subject>Beverages</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cardiovascular diseases</subject><subject>Cascades</subject><subject>Cell cycle</subject><subject>Citrus paradisi</subject><subject>Cluster Analysis</subject><subject>Coding</subject><subject>Coffee</subject><subject>Coronary vessels</subject><subject>Diet</subject><subject>Dietary supplements</subject><subject>Diseases</subject><subject>DNA binding proteins</subject><subject>DNA microarrays</subject><subject>Down-Regulation - drug effects</subject><subject>Down-Regulation - genetics</subject><subject>Epidemiology</subject><subject>Fatty acids</subject><subject>Flavonoids</subject><subject>Food</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Heart diseases</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Modulation</subject><subject>Mutation</subject><subject>Nervous system diseases</subject><subject>Neurodegenerative diseases</subject><subject>Neurological diseases</subject><subject>Neutrophils</subject><subject>Non-coding RNA</subject><subject>Nutrition</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oxidative stress</subject><subject>Pathogenesis</subject><subject>Pathways</subject><subject>Polyphenols</subject><subject>Polyphenols - pharmacology</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA polymerase</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Rodents</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Signaling</subject><subject>Supplementation</subject><subject>Target recognition</subject><subject>Tea</subject><subject>Transcription (Genetics)</subject><subject>Transcription factors</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - genetics</subject><subject>Wine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1-L1DAUxYso7rr6DUQDgrAPM7ZJmjQ-CMOyugOjC-uf15Amt9MMnaY27brjpzd1OstUFKQPLbe_cy73JDeKnifxPCE8ebNxfVurat64GuZxjEVG-IPoNBEEzxiOycOj75PoifebOE5Jxtjj6ARjnBIu-Gn086MzfaU662rkCrS1N58WCO6aFrwfavkOGQudaneocdWuKaF2lUe2Rqpxl8hAYbWFugtKDW-RQjX8QFvQpaqt3w6WXQlI6UODI5On0aNCVR6eje-z6Ov7yy8XV7PV9YflxWI101wk3SzFOuF5ZighgoV-mKWCcsLzmGpFTM40EUAoBZbmLKNEU8pYlmgjqCl0jslZ9HLv21TOyzE2LxOCMeUhtCwQyz1hnNrIprXbMK90ysrfBdeupWo7qyuQCmOWp0VGdFBnqcmEzk2RYghRC6aS4PVu7NbnWzA6ZNOqamI6_VPbUq7drSQ4pYwMBud7g_IP2dViJYdaHBKIeUZvB_bV2Kx133vw3T_GG6m1ChPYunChsd5ar-WCcp4wwclAzf9ChcdAONtwyQob6hPB-UQQmA7uurXqvZfLzzf_z15_m7Kvj9gSVNWV3lX9cIX8FKR7ULfO-xaK-7iSWA47ckhDDjsixx0JshfHJ3QvOiwF-QW0AgwJ</recordid><startdate>20120110</startdate><enddate>20120110</enddate><creator>Milenkovic, Dragan</creator><creator>Deval, Christiane</creator><creator>Gouranton, Erwan</creator><creator>Landrier, Jean-François</creator><creator>Scalbert, Augustin</creator><creator>Morand, Christine</creator><creator>Mazur, Andrzej</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1067-5066</orcidid><orcidid>https://orcid.org/0000-0001-6353-0912</orcidid><orcidid>https://orcid.org/0000-0001-6651-6710</orcidid><orcidid>https://orcid.org/0000-0001-8128-1032</orcidid><orcidid>https://orcid.org/0000-0002-8690-8014</orcidid></search><sort><creationdate>20120110</creationdate><title>Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols</title><author>Milenkovic, Dragan ; Deval, Christiane ; Gouranton, Erwan ; Landrier, Jean-François ; Scalbert, Augustin ; Morand, Christine ; Mazur, Andrzej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c791t-52c17b8d43396def26594737b04ca3db6c39e344e65b6843c446681cd94dfcb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actin Cytoskeleton - drug effects</topic><topic>Actin Cytoskeleton - genetics</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants (Nutrients)</topic><topic>Apolipoprotein E</topic><topic>Apolipoproteins</topic><topic>Apolipoproteins E - deficiency</topic><topic>Apolipoproteins E - metabolism</topic><topic>Apoptosis</topic><topic>Arabidopsis thaliana</topic><topic>Atherosclerosis</topic><topic>Beverages</topic><topic>Biology</topic><topic>Cancer</topic><topic>Cardiovascular diseases</topic><topic>Cascades</topic><topic>Cell cycle</topic><topic>Citrus paradisi</topic><topic>Cluster Analysis</topic><topic>Coding</topic><topic>Coffee</topic><topic>Coronary vessels</topic><topic>Diet</topic><topic>Dietary supplements</topic><topic>Diseases</topic><topic>DNA binding proteins</topic><topic>DNA microarrays</topic><topic>Down-Regulation - drug effects</topic><topic>Down-Regulation - genetics</topic><topic>Epidemiology</topic><topic>Fatty acids</topic><topic>Flavonoids</topic><topic>Food</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Heart diseases</topic><topic>Humans</topic><topic>In vivo methods and tests</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Modulation</topic><topic>Mutation</topic><topic>Nervous system diseases</topic><topic>Neurodegenerative diseases</topic><topic>Neurological diseases</topic><topic>Neutrophils</topic><topic>Non-coding RNA</topic><topic>Nutrition</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oxidative stress</topic><topic>Pathogenesis</topic><topic>Pathways</topic><topic>Polyphenols</topic><topic>Polyphenols - pharmacology</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA polymerase</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Rodents</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Signaling</topic><topic>Supplementation</topic><topic>Target recognition</topic><topic>Tea</topic><topic>Transcription (Genetics)</topic><topic>Transcription factors</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - genetics</topic><topic>Wine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milenkovic, Dragan</creatorcontrib><creatorcontrib>Deval, Christiane</creatorcontrib><creatorcontrib>Gouranton, Erwan</creatorcontrib><creatorcontrib>Landrier, Jean-François</creatorcontrib><creatorcontrib>Scalbert, Augustin</creatorcontrib><creatorcontrib>Morand, Christine</creatorcontrib><creatorcontrib>Mazur, Andrzej</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale in Context : Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milenkovic, Dragan</au><au>Deval, Christiane</au><au>Gouranton, Erwan</au><au>Landrier, Jean-François</au><au>Scalbert, Augustin</au><au>Morand, Christine</au><au>Mazur, Andrzej</au><au>Krahe, Ralf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-01-10</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>e29837</spage><epage>167</epage><pages>e29837-167</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Polyphenols are the most abundant antioxidants in the human diet and are widespread constituents of fruits and beverages, such as tea, coffee or wine. Epidemiological, clinical and animal studies support a role of polyphenols in the prevention of various diseases, such as cardiovascular diseases, cancers or neurodegenerative diseases. Recent findings suggest that polyphenols could interact with cellular signaling cascades regulating the activity of transcription factors and consequently affecting the expression of genes. However, the impact of polyphenol on the expression of microRNA, small non-coding RNAs, has not yet been studied. The aim of this study was to investigate the impact of dietary supplementation with polyphenols at nutritional doses on miRNA expression in the livers of apolipoprotein E-deficient mice (apoE⁻/⁻) jointly with mRNA expression profiling.
Using microarrays, we measured the global miRNA expression in the livers of wild-type (C57B6/J) mice or apoE⁻/⁻ mice fed diets supplemented with one of nine different polyphenols or a control diet. This analysis revealed that knock-out of the apoE gene induced significant modulation in the expression of miRNA. Moreover, changes in miRNA expression were observed after polyphenol supplementation, and five miRNAs (mmu-miR-291b-5p, mmu-miR-296-5p, mmu-miR-30c-1*, mmu-miR-467b* and mmu-miR-374*) were identified as being commonly modulated by these polyphenols. We also observed that these polyphenols counteracted the modulation of miRNA expression induced by apoE mutation. Pathway analyses on these five miRNA-target genes revealed common pathways, some of which were also identified from a pathway analysis on mRNA profiles.
This in vivo study demonstrated for the first time that polyphenols at nutritional doses modulate the expression of miRNA in the liver. Even if structurally different, all polyphenols induced a similar miRNA expression profile. Common pathways were identified from both miRNA-target and mRNA analysis, revealing cellular functions that could be regulated by polyphenols at both the miRNA and mRNA level.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22253797</pmid><doi>10.1371/journal.pone.0029837</doi><tpages>e29837</tpages><orcidid>https://orcid.org/0000-0002-1067-5066</orcidid><orcidid>https://orcid.org/0000-0001-6353-0912</orcidid><orcidid>https://orcid.org/0000-0001-6651-6710</orcidid><orcidid>https://orcid.org/0000-0001-8128-1032</orcidid><orcidid>https://orcid.org/0000-0002-8690-8014</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2012-01, Vol.7 (1), p.e29837-167 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1322478378 |
source | PubMed (Medline); Publicly Available Content Database |
subjects | Actin Cytoskeleton - drug effects Actin Cytoskeleton - genetics Analysis Animals Antioxidants Antioxidants (Nutrients) Apolipoprotein E Apolipoproteins Apolipoproteins E - deficiency Apolipoproteins E - metabolism Apoptosis Arabidopsis thaliana Atherosclerosis Beverages Biology Cancer Cardiovascular diseases Cascades Cell cycle Citrus paradisi Cluster Analysis Coding Coffee Coronary vessels Diet Dietary supplements Diseases DNA binding proteins DNA microarrays Down-Regulation - drug effects Down-Regulation - genetics Epidemiology Fatty acids Flavonoids Food Gene expression Gene Expression Profiling Gene Expression Regulation - drug effects Genes Genetic research Heart diseases Humans In vivo methods and tests Laboratories Life Sciences Liver Liver - drug effects Liver - metabolism Medical research Medicine Metabolites Mice Mice, Inbred C57BL MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Modulation Mutation Nervous system diseases Neurodegenerative diseases Neurological diseases Neutrophils Non-coding RNA Nutrition Oligonucleotide Array Sequence Analysis Oxidative stress Pathogenesis Pathways Polyphenols Polyphenols - pharmacology Proteins Ribonucleic acid RNA RNA polymerase RNA, Messenger - genetics RNA, Messenger - metabolism Rodents Signal Transduction - drug effects Signal Transduction - genetics Signaling Supplementation Target recognition Tea Transcription (Genetics) Transcription factors Up-Regulation - drug effects Up-Regulation - genetics Wine |
title | Modulation of miRNA expression by dietary polyphenols in apoE deficient mice: a new mechanism of the action of polyphenols |
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