TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma

Metastasis is a multistep process and the main cause of mortality in lung cancer patients. We previously showed that EGFR mutations were associated with a copy number gain at a locus encompassing the TWIST1 gene on chromosome 7. TWIST1 is a highly conserved developmental gene involved in embryogenes...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-01, Vol.7 (1), p.e29954-e29954
Main Authors: Pallier, Karine, Cessot, Anatole, Côté, Jean-Francois, Just, Pierre-Alexandre, Cazes, Aurélie, Fabre, Elizabeth, Danel, Claire, Riquet, Marc, Devouassoux-Shisheboran, Mojgan, Ansieau, Stéphane, Puisieux, Alain, Laurent-Puig, Pierre, Blons, Hélène
Format: Article
Language:English
Subjects:
Activation
Adenocarcinoma
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Aged
Aged, 80 and over
Antigens, CD - genetics
Antigens, CD - metabolism
Biology
Blotting, Western
Cadherins - genetics
Cadherins - metabolism
Cancer
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Chromosome 7
Cooperation
Copy number
Development and progression
E-cadherin
Embryogenesis
Embryonic growth stage
Epidermal growth factor
Epidermal Growth Factor - pharmacology
Epidermal growth factor receptors
Epidermal growth factors
Epithelial-Mesenchymal Transition - drug effects
Epithelial-Mesenchymal Transition - genetics
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genetic aspects
Head & neck cancer
Health aspects
Humans
Hypoxia
Immunohistochemistry
Kaplan-Meier Estimate
Kinases
Lung cancer
Lung diseases
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Medicine
Mesenchyme
Metastases
Metastasis
Middle Aged
Mortality
Motility
Mutation
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Pathology
Patients
Receptor, Epidermal Growth Factor - genetics
Receptor, Epidermal Growth Factor - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
Senescence
Smoking
Stem cells
Surgery
Thoracic surgery
Tumor cell lines
Tumors
Twist-Related Protein 1 - genetics
Twist-Related Protein 1 - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093
cites cdi_FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093
container_end_page e29954
container_issue 1
container_start_page e29954
container_title PloS one
container_volume 7
creator Pallier, Karine
Cessot, Anatole
Côté, Jean-Francois
Just, Pierre-Alexandre
Cazes, Aurélie
Fabre, Elizabeth
Danel, Claire
Riquet, Marc
Devouassoux-Shisheboran, Mojgan
Ansieau, Stéphane
Puisieux, Alain
Laurent-Puig, Pierre
Blons, Hélène
description Metastasis is a multistep process and the main cause of mortality in lung cancer patients. We previously showed that EGFR mutations were associated with a copy number gain at a locus encompassing the TWIST1 gene on chromosome 7. TWIST1 is a highly conserved developmental gene involved in embryogenesis that may be reactivated in cancers promoting both malignant conversion and cancer progression through an epithelial to mesenchymal transition (EMT). The aim of this study was to investigate the possible implication of TWIST1 reactivation on the acquisition of a mesenchymal phenotype in EGFR mutated lung cancer. We studied a series of consecutive lung adenocarcinoma from Caucasian non-smokers for which surgical frozen samples were available (n = 33) and showed that TWIST1 expression was linked to EGFR mutations (P
doi_str_mv 10.1371/journal.pone.0029954
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1323077581</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477075497</galeid><doaj_id>oai_doaj_org_article_0d3acebae6dd446db855594dedf8253d</doaj_id><sourcerecordid>A477075497</sourcerecordid><originalsourceid>FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093</originalsourceid><addsrcrecordid>eNqNk99v0zAQxyMEYmPwHyCwhATiocXxz-QFaZq2UWnSpK3Ao-XYl9ZVYpfYAfbfk9BsatEekB9snz_3Pft8l2WvczzPqcw_bULfed3Mt8HDHGNSlpw9yY7zkpKZIJg-3VsfZS9i3GDMaSHE8-yIECIJEew4s8vvi9tljjTy8AtZSNC1zmufUKgRbF1aQ-N0g1JALUTwZn3XjttO--iSCx45j84vL25Q2yedwKKm9yukLfhgdGecD61-mT2rdRPh1TSfZF8vzpdnX2ZX15eLs9OrmZFcplmliwrTglKKjaVgbCUMLQkDQRitCiN1LQumqSwLAVVtmbR5RQQHyWltcUlPsrc73W0TopoSFFVOCcVS8iIfiMWOsEFv1LZzre7uVNBO_TWEbqV0l5xpQGFLtYFKg7CWMWGrgnNeMgu2LgindtD6PEXrqxasAT9kpTkQPTzxbq1W4aeiROC8kIPAh0mgCz96iEm1LhpoGu0h9FGVuSy44FIM5Lt_yMcfN1ErPdzf-ToMYc2oqU6ZlFhyVo5R549Qw7DQOjMUU-0G-4HDxwOHgUnwO610H6Na3N78P3v97ZB9v8euQTdpHUPTj1UVD0G2A00XYuygfshxjtXYC_fZUGMvqKkXBrc3-__z4HRf_PQPGOME2A</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1323077581</pqid></control><display><type>article</type><title>TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Pallier, Karine ; Cessot, Anatole ; Côté, Jean-Francois ; Just, Pierre-Alexandre ; Cazes, Aurélie ; Fabre, Elizabeth ; Danel, Claire ; Riquet, Marc ; Devouassoux-Shisheboran, Mojgan ; Ansieau, Stéphane ; Puisieux, Alain ; Laurent-Puig, Pierre ; Blons, Hélène</creator><contributor>Klymkowsky, Michael</contributor><creatorcontrib>Pallier, Karine ; Cessot, Anatole ; Côté, Jean-Francois ; Just, Pierre-Alexandre ; Cazes, Aurélie ; Fabre, Elizabeth ; Danel, Claire ; Riquet, Marc ; Devouassoux-Shisheboran, Mojgan ; Ansieau, Stéphane ; Puisieux, Alain ; Laurent-Puig, Pierre ; Blons, Hélène ; Klymkowsky, Michael</creatorcontrib><description>Metastasis is a multistep process and the main cause of mortality in lung cancer patients. We previously showed that EGFR mutations were associated with a copy number gain at a locus encompassing the TWIST1 gene on chromosome 7. TWIST1 is a highly conserved developmental gene involved in embryogenesis that may be reactivated in cancers promoting both malignant conversion and cancer progression through an epithelial to mesenchymal transition (EMT). The aim of this study was to investigate the possible implication of TWIST1 reactivation on the acquisition of a mesenchymal phenotype in EGFR mutated lung cancer. We studied a series of consecutive lung adenocarcinoma from Caucasian non-smokers for which surgical frozen samples were available (n = 33) and showed that TWIST1 expression was linked to EGFR mutations (P&lt;0.001), to low CDH1 expression (P&lt;0.05) and low disease free survival (P = 0.044). To validate that TWIST1 is a driver of EMT in EGFR mutated lung cancer, we used five human lung cancer cell lines and demonstrated that EMT and the associated cell mobility were dependent upon TWIST1 expression in cells with EGFR mutation. Moreover a decrease of EGFR pathway stimulation through EGF retrieval or an inhibition of TWIST1 expression by small RNA technology reversed the phenomenon. Collectively, our in vivo and in vitro findings support that TWIST1 collaborates with the EGF pathway in promoting EMT in EGFR mutated lung adenocarcinoma and that large series of EGFR mutated lung cancer patients are needed to further define the prognostic role of TWIST1 reactivation in this subgroup.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0029954</identifier><identifier>PMID: 22272264</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Adenocarcinoma ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Aged ; Aged, 80 and over ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biology ; Blotting, Western ; Cadherins - genetics ; Cadherins - metabolism ; Cancer ; Cell growth ; Cell Line, Tumor ; Cell Movement - genetics ; Chromosome 7 ; Cooperation ; Copy number ; Development and progression ; E-cadherin ; Embryogenesis ; Embryonic growth stage ; Epidermal growth factor ; Epidermal Growth Factor - pharmacology ; Epidermal growth factor receptors ; Epidermal growth factors ; Epithelial-Mesenchymal Transition - drug effects ; Epithelial-Mesenchymal Transition - genetics ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Head &amp; neck cancer ; Health aspects ; Humans ; Hypoxia ; Immunohistochemistry ; Kaplan-Meier Estimate ; Kinases ; Lung cancer ; Lung diseases ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Male ; Medicine ; Mesenchyme ; Metastases ; Metastasis ; Middle Aged ; Mortality ; Motility ; Mutation ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Pathology ; Patients ; Receptor, Epidermal Growth Factor - genetics ; Receptor, Epidermal Growth Factor - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA Interference ; Senescence ; Smoking ; Stem cells ; Surgery ; Thoracic surgery ; Tumor cell lines ; Tumors ; Twist-Related Protein 1 - genetics ; Twist-Related Protein 1 - metabolism</subject><ispartof>PloS one, 2012-01, Vol.7 (1), p.e29954-e29954</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Pallier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Pallier et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093</citedby><cites>FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1323077581/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1323077581?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22272264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Klymkowsky, Michael</contributor><creatorcontrib>Pallier, Karine</creatorcontrib><creatorcontrib>Cessot, Anatole</creatorcontrib><creatorcontrib>Côté, Jean-Francois</creatorcontrib><creatorcontrib>Just, Pierre-Alexandre</creatorcontrib><creatorcontrib>Cazes, Aurélie</creatorcontrib><creatorcontrib>Fabre, Elizabeth</creatorcontrib><creatorcontrib>Danel, Claire</creatorcontrib><creatorcontrib>Riquet, Marc</creatorcontrib><creatorcontrib>Devouassoux-Shisheboran, Mojgan</creatorcontrib><creatorcontrib>Ansieau, Stéphane</creatorcontrib><creatorcontrib>Puisieux, Alain</creatorcontrib><creatorcontrib>Laurent-Puig, Pierre</creatorcontrib><creatorcontrib>Blons, Hélène</creatorcontrib><title>TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Metastasis is a multistep process and the main cause of mortality in lung cancer patients. We previously showed that EGFR mutations were associated with a copy number gain at a locus encompassing the TWIST1 gene on chromosome 7. TWIST1 is a highly conserved developmental gene involved in embryogenesis that may be reactivated in cancers promoting both malignant conversion and cancer progression through an epithelial to mesenchymal transition (EMT). The aim of this study was to investigate the possible implication of TWIST1 reactivation on the acquisition of a mesenchymal phenotype in EGFR mutated lung cancer. We studied a series of consecutive lung adenocarcinoma from Caucasian non-smokers for which surgical frozen samples were available (n = 33) and showed that TWIST1 expression was linked to EGFR mutations (P&lt;0.001), to low CDH1 expression (P&lt;0.05) and low disease free survival (P = 0.044). To validate that TWIST1 is a driver of EMT in EGFR mutated lung cancer, we used five human lung cancer cell lines and demonstrated that EMT and the associated cell mobility were dependent upon TWIST1 expression in cells with EGFR mutation. Moreover a decrease of EGFR pathway stimulation through EGF retrieval or an inhibition of TWIST1 expression by small RNA technology reversed the phenomenon. Collectively, our in vivo and in vitro findings support that TWIST1 collaborates with the EGF pathway in promoting EMT in EGFR mutated lung adenocarcinoma and that large series of EGFR mutated lung cancer patients are needed to further define the prognostic role of TWIST1 reactivation in this subgroup.</description><subject>Activation</subject><subject>Adenocarcinoma</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biology</subject><subject>Blotting, Western</subject><subject>Cadherins - genetics</subject><subject>Cadherins - metabolism</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Chromosome 7</subject><subject>Cooperation</subject><subject>Copy number</subject><subject>Development and progression</subject><subject>E-cadherin</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Epidermal growth factor</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Epidermal growth factor receptors</subject><subject>Epidermal growth factors</subject><subject>Epithelial-Mesenchymal Transition - drug effects</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Head &amp; neck cancer</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hypoxia</subject><subject>Immunohistochemistry</subject><subject>Kaplan-Meier Estimate</subject><subject>Kinases</subject><subject>Lung cancer</subject><subject>Lung diseases</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Medicine</subject><subject>Mesenchyme</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Mortality</subject><subject>Motility</subject><subject>Mutation</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Pathology</subject><subject>Patients</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>Senescence</subject><subject>Smoking</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Thoracic surgery</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>Twist-Related Protein 1 - genetics</subject><subject>Twist-Related Protein 1 - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99v0zAQxyMEYmPwHyCwhATiocXxz-QFaZq2UWnSpK3Ao-XYl9ZVYpfYAfbfk9BsatEekB9snz_3Pft8l2WvczzPqcw_bULfed3Mt8HDHGNSlpw9yY7zkpKZIJg-3VsfZS9i3GDMaSHE8-yIECIJEew4s8vvi9tljjTy8AtZSNC1zmufUKgRbF1aQ-N0g1JALUTwZn3XjttO--iSCx45j84vL25Q2yedwKKm9yukLfhgdGecD61-mT2rdRPh1TSfZF8vzpdnX2ZX15eLs9OrmZFcplmliwrTglKKjaVgbCUMLQkDQRitCiN1LQumqSwLAVVtmbR5RQQHyWltcUlPsrc73W0TopoSFFVOCcVS8iIfiMWOsEFv1LZzre7uVNBO_TWEbqV0l5xpQGFLtYFKg7CWMWGrgnNeMgu2LgindtD6PEXrqxasAT9kpTkQPTzxbq1W4aeiROC8kIPAh0mgCz96iEm1LhpoGu0h9FGVuSy44FIM5Lt_yMcfN1ErPdzf-ToMYc2oqU6ZlFhyVo5R549Qw7DQOjMUU-0G-4HDxwOHgUnwO610H6Na3N78P3v97ZB9v8euQTdpHUPTj1UVD0G2A00XYuygfshxjtXYC_fZUGMvqKkXBrc3-__z4HRf_PQPGOME2A</recordid><startdate>20120117</startdate><enddate>20120117</enddate><creator>Pallier, Karine</creator><creator>Cessot, Anatole</creator><creator>Côté, Jean-Francois</creator><creator>Just, Pierre-Alexandre</creator><creator>Cazes, Aurélie</creator><creator>Fabre, Elizabeth</creator><creator>Danel, Claire</creator><creator>Riquet, Marc</creator><creator>Devouassoux-Shisheboran, Mojgan</creator><creator>Ansieau, Stéphane</creator><creator>Puisieux, Alain</creator><creator>Laurent-Puig, Pierre</creator><creator>Blons, Hélène</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120117</creationdate><title>TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma</title><author>Pallier, Karine ; Cessot, Anatole ; Côté, Jean-Francois ; Just, Pierre-Alexandre ; Cazes, Aurélie ; Fabre, Elizabeth ; Danel, Claire ; Riquet, Marc ; Devouassoux-Shisheboran, Mojgan ; Ansieau, Stéphane ; Puisieux, Alain ; Laurent-Puig, Pierre ; Blons, Hélène</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Activation</topic><topic>Adenocarcinoma</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biology</topic><topic>Blotting, Western</topic><topic>Cadherins - genetics</topic><topic>Cadherins - metabolism</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Chromosome 7</topic><topic>Cooperation</topic><topic>Copy number</topic><topic>Development and progression</topic><topic>E-cadherin</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Epidermal growth factor</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Epidermal growth factor receptors</topic><topic>Epidermal growth factors</topic><topic>Epithelial-Mesenchymal Transition - drug effects</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Head &amp; neck cancer</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Hypoxia</topic><topic>Immunohistochemistry</topic><topic>Kaplan-Meier Estimate</topic><topic>Kinases</topic><topic>Lung cancer</topic><topic>Lung diseases</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Medicine</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Mortality</topic><topic>Motility</topic><topic>Mutation</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Pathology</topic><topic>Patients</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>Senescence</topic><topic>Smoking</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Thoracic surgery</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><topic>Twist-Related Protein 1 - genetics</topic><topic>Twist-Related Protein 1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pallier, Karine</creatorcontrib><creatorcontrib>Cessot, Anatole</creatorcontrib><creatorcontrib>Côté, Jean-Francois</creatorcontrib><creatorcontrib>Just, Pierre-Alexandre</creatorcontrib><creatorcontrib>Cazes, Aurélie</creatorcontrib><creatorcontrib>Fabre, Elizabeth</creatorcontrib><creatorcontrib>Danel, Claire</creatorcontrib><creatorcontrib>Riquet, Marc</creatorcontrib><creatorcontrib>Devouassoux-Shisheboran, Mojgan</creatorcontrib><creatorcontrib>Ansieau, Stéphane</creatorcontrib><creatorcontrib>Puisieux, Alain</creatorcontrib><creatorcontrib>Laurent-Puig, Pierre</creatorcontrib><creatorcontrib>Blons, Hélène</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Science (Gale in Context)</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Agricultural &amp; Environmental Science</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pallier, Karine</au><au>Cessot, Anatole</au><au>Côté, Jean-Francois</au><au>Just, Pierre-Alexandre</au><au>Cazes, Aurélie</au><au>Fabre, Elizabeth</au><au>Danel, Claire</au><au>Riquet, Marc</au><au>Devouassoux-Shisheboran, Mojgan</au><au>Ansieau, Stéphane</au><au>Puisieux, Alain</au><au>Laurent-Puig, Pierre</au><au>Blons, Hélène</au><au>Klymkowsky, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-01-17</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>e29954</spage><epage>e29954</epage><pages>e29954-e29954</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Metastasis is a multistep process and the main cause of mortality in lung cancer patients. We previously showed that EGFR mutations were associated with a copy number gain at a locus encompassing the TWIST1 gene on chromosome 7. TWIST1 is a highly conserved developmental gene involved in embryogenesis that may be reactivated in cancers promoting both malignant conversion and cancer progression through an epithelial to mesenchymal transition (EMT). The aim of this study was to investigate the possible implication of TWIST1 reactivation on the acquisition of a mesenchymal phenotype in EGFR mutated lung cancer. We studied a series of consecutive lung adenocarcinoma from Caucasian non-smokers for which surgical frozen samples were available (n = 33) and showed that TWIST1 expression was linked to EGFR mutations (P&lt;0.001), to low CDH1 expression (P&lt;0.05) and low disease free survival (P = 0.044). To validate that TWIST1 is a driver of EMT in EGFR mutated lung cancer, we used five human lung cancer cell lines and demonstrated that EMT and the associated cell mobility were dependent upon TWIST1 expression in cells with EGFR mutation. Moreover a decrease of EGFR pathway stimulation through EGF retrieval or an inhibition of TWIST1 expression by small RNA technology reversed the phenomenon. Collectively, our in vivo and in vitro findings support that TWIST1 collaborates with the EGF pathway in promoting EMT in EGFR mutated lung adenocarcinoma and that large series of EGFR mutated lung cancer patients are needed to further define the prognostic role of TWIST1 reactivation in this subgroup.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22272264</pmid><doi>10.1371/journal.pone.0029954</doi><tpages>e29954</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2012-01, Vol.7 (1), p.e29954-e29954
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1323077581
source Publicly Available Content (ProQuest); PubMed Central
subjects Activation
Adenocarcinoma
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Aged
Aged, 80 and over
Antigens, CD - genetics
Antigens, CD - metabolism
Biology
Blotting, Western
Cadherins - genetics
Cadherins - metabolism
Cancer
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Chromosome 7
Cooperation
Copy number
Development and progression
E-cadherin
Embryogenesis
Embryonic growth stage
Epidermal growth factor
Epidermal Growth Factor - pharmacology
Epidermal growth factor receptors
Epidermal growth factors
Epithelial-Mesenchymal Transition - drug effects
Epithelial-Mesenchymal Transition - genetics
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genetic aspects
Head & neck cancer
Health aspects
Humans
Hypoxia
Immunohistochemistry
Kaplan-Meier Estimate
Kinases
Lung cancer
Lung diseases
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Male
Medicine
Mesenchyme
Metastases
Metastasis
Middle Aged
Mortality
Motility
Mutation
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Pathology
Patients
Receptor, Epidermal Growth Factor - genetics
Receptor, Epidermal Growth Factor - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
Senescence
Smoking
Stem cells
Surgery
Thoracic surgery
Tumor cell lines
Tumors
Twist-Related Protein 1 - genetics
Twist-Related Protein 1 - metabolism
title TWIST1 a new determinant of epithelial to mesenchymal transition in EGFR mutated lung adenocarcinoma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T15%3A15%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TWIST1%20a%20new%20determinant%20of%20epithelial%20to%20mesenchymal%20transition%20in%20EGFR%20mutated%20lung%20adenocarcinoma&rft.jtitle=PloS%20one&rft.au=Pallier,%20Karine&rft.date=2012-01-17&rft.volume=7&rft.issue=1&rft.spage=e29954&rft.epage=e29954&rft.pages=e29954-e29954&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0029954&rft_dat=%3Cgale_plos_%3EA477075497%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c757t-ba8b0383330cd3ecdb6c3924e6243b8c7af784a37986ebfd47d1b265e753fd093%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1323077581&rft_id=info:pmid/22272264&rft_galeid=A477075497&rfr_iscdi=true