EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle

• Published in: PloS one 2012-02, Vol.7 (2), p.e31054-e31054
• Main Authors: Jensen, Thomas E, Leutert, Robin, Rasmussen, Søren T, Mouatt, Joshua R, Christiansen, Mette L B, Jensen, Bente R, Richter, Erik A
• Format: Article
• Language: English
• Subjects: Activation; Adenosine triphosphatase; Biology; Biosynthesis; Catalase; Contraction; Electromyography; EMG; Enzyme Activation; Enzymes; Exercise; Extracellular signal-regulated kinase; Fitness training programs; Gastrocnemius muscle; Gene expression; Glucose; Glucose Transporter Type 4 - metabolism; Glycogen; Hexokinase; Hexokinase - metabolism; Humans; Kinases; Medicine; Mice; Mitochondria; Muscle contraction; Muscle, Skeletal - enzymology; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Muscles; Musculoskeletal system; Mutation; Oxygen; Oxygen consumption; Phosphatase; Phosphorylation; Physiology; Protein expression; Protein Kinases - metabolism; Proteins; Reactive oxygen species; Rodents; Signal Transduction; Signaling; Skeletal muscle; Soleus muscle; Walking
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0031054

In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles with high mitochondrial content, possibly related to differential reactive oxygen species (ROS)-scavenging ability or resting glycogen content. To evaluate these parameters in humans, biopsies from soleus, gastrocnemius and vastus lateralis muscles were taken before and after a 45 min inclined (15%) walking exercise bout at 69% VO2(max) aimed at simultaneously activating soleus and gastrocnemius in a comparable dynamic work-pattern. Hexokinase II and GLUT4 were 46-59% and 26-38% higher (p