Automated whole animal bio-imaging assay for human cancer dissemination

A quantitative bio-imaging platform is developed for analysis of human cancer dissemination in a short-term vertebrate xenotransplantation assay. Six days after implantation of cancer cells in zebrafish embryos, automated imaging in 96 well plates coupled to image analysis algorithms quantifies spre...

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Bibliographic Details
Published in:PloS one 2012-02, Vol.7 (2), p.e31281
Main Authors: Ghotra, Veerander P S, He, Shuning, de Bont, Hans, van der Ent, Wietske, Spaink, Herman P, van de Water, Bob, Snaar-Jagalska, B Ewa, Danen, Erik H J
Format: Article
Language:English
Subjects:
Algorithms
Androgens
Animal models
Animals
Assaying
Biology
Breast cancer
Breast Neoplasms - pathology
Cancer
Cancer metastasis
Cancer screening
Cell Line, Tumor
Colorectal Neoplasms - pathology
Danio rerio
Diagnostic Imaging - methods
Drug Discovery - methods
E-cadherin
Embryos
Female
Humans
Image analysis
Image processing
Imaging
Implantation
Male
Medicine
Mesenchyme
Metastases
Microarray Analysis
Neoplasm Transplantation
Neoplasms - pathology
Prostate cancer
Prostatic Neoplasms - pathology
Ribonucleic acid
RNA
RNA-mediated interference
Rodents
Surgical implants
Transplantation, Heterologous
Tumors
Xenografts
Xenotransplantation
Zebrafish
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Summary:A quantitative bio-imaging platform is developed for analysis of human cancer dissemination in a short-term vertebrate xenotransplantation assay. Six days after implantation of cancer cells in zebrafish embryos, automated imaging in 96 well plates coupled to image analysis algorithms quantifies spreading throughout the host. Findings in this model correlate with behavior in long-term rodent xenograft models for panels of poorly- versus highly malignant cell lines derived from breast, colorectal, and prostate cancer. In addition, cancer cells with scattered mesenchymal characteristics show higher dissemination capacity than cell types with epithelial appearance. Moreover, RNA interference establishes the metastasis-suppressor role for E-cadherin in this model. This automated quantitative whole animal bio-imaging assay can serve as a first-line in vivo screening step in the anti-cancer drug target discovery pipeline.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0031281