The transcriptome of compatible and incompatible interactions of potato (Solanum tuberosum) with Phytophthora infestans revealed by DeepSAGE analysis

Late blight, caused by the oomycete Phytophthora infestans, is the most important disease of potato (Solanum tuberosum). Understanding the molecular basis of resistance and susceptibility to late blight is therefore highly relevant for developing resistant cultivars, either by marker-assissted selec...

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Published in:PloS one 2012-02, Vol.7 (2), p.e31526-e31526
Main Authors: Gyetvai, Gabor, Sønderkær, Mads, Göbel, Ulrike, Basekow, Rico, Ballvora, Agim, Imhoff, Maren, Kersten, Birgit, Nielsen, Kåre-Lehman, Gebhardt, Christiane
Format: Article
Language:English
Subjects:
Agriculture
Analysis
Arabidopsis
Binding sites
Biological properties
Biological samples
Biology
Blight
Carbon dioxide
Carbon dioxide fixation
Carbon sequestration
Compatibility
Consortia
Cultivars
Environmental engineering
Gene expression
Gene frequency
Genes
Genetic aspects
Genetic engineering
Genomes
Genotypes
Health aspects
Host plants
Infections
Late blight
Ontology
Oomycetes
Pathogens
Photosynthesis
Physiology
Phytophthora
Phytophthora infestans
Phytophthora infestans - genetics
Phytophthora infestans - pathogenicity
Plant diseases
Plant Diseases - parasitology
Plant genetics
Plants (botany)
Polymerase chain reaction
Potatoes
Solanum tuberosum
Solanum tuberosum - microbiology
Tags
Transcription
Transcription (Genetics)
Transcriptome - genetics
Transgenic plants
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cited_by cdi_FETCH-LOGICAL-c625t-859b76b80b1cd9c6730b14462cc15e162acfcaa668dec7d62dc8ce7f8cd0c2e33
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container_issue 2
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container_title PloS one
container_volume 7
creator Gyetvai, Gabor
Sønderkær, Mads
Göbel, Ulrike
Basekow, Rico
Ballvora, Agim
Imhoff, Maren
Kersten, Birgit
Nielsen, Kåre-Lehman
Gebhardt, Christiane
description Late blight, caused by the oomycete Phytophthora infestans, is the most important disease of potato (Solanum tuberosum). Understanding the molecular basis of resistance and susceptibility to late blight is therefore highly relevant for developing resistant cultivars, either by marker-assissted selection or by transgenic approaches. Specific P. infestans races having the Avr1 effector gene trigger a hypersensitive resistance response in potato plants carrying the R1 resistance gene (incompatible interaction) and cause disease in plants lacking R1 (compatible interaction). The transcriptomes of the compatible and incompatible interaction were captured by DeepSAGE analysis of 44 biological samples comprising five genotypes, differing only by the presence or absence of the R1 transgene, three infection time points and three biological replicates. 30,859 unique 21 base pair sequence tags were obtained, one third of which did not match any known potato transcript sequence. Two third of the tags were expressed at low frequency (
doi_str_mv 10.1371/journal.pone.0031526
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Two third of the tags were expressed at low frequency (&lt;10 tag counts/million). 20,470 unitags matched to approximately twelve thousand potato transcribed genes. Tag frequencies were compared between compatible and incompatible interactions over the infection time course and between compatible and incompatible genotypes. Transcriptional changes were more numerous in compatible than in incompatible interactions. In contrast to incompatible interactions, transcriptional changes in the compatible interaction were observed predominantly for multigene families encoding defense response genes and genes functional in photosynthesis and CO(2) fixation. Numerous transcriptional differences were also observed between near isogenic genotypes prior to infection with P. infestans. 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1932-6203
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subjects Agriculture
Analysis
Arabidopsis
Binding sites
Biological properties
Biological samples
Biology
Blight
Carbon dioxide
Carbon dioxide fixation
Carbon sequestration
Compatibility
Consortia
Cultivars
Environmental engineering
Gene expression
Gene frequency
Genes
Genetic aspects
Genetic engineering
Genomes
Genotypes
Health aspects
Host plants
Infections
Late blight
Ontology
Oomycetes
Pathogens
Photosynthesis
Physiology
Phytophthora
Phytophthora infestans
Phytophthora infestans - genetics
Phytophthora infestans - pathogenicity
Plant diseases
Plant Diseases - parasitology
Plant genetics
Plants (botany)
Polymerase chain reaction
Potatoes
Solanum tuberosum
Solanum tuberosum - microbiology
Tags
Transcription
Transcription (Genetics)
Transcriptome - genetics
Transgenic plants
title The transcriptome of compatible and incompatible interactions of potato (Solanum tuberosum) with Phytophthora infestans revealed by DeepSAGE analysis
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