Differential expression profile and genetic variants of microRNAs sequences in breast cancer patients

The technology available for cancer diagnosis and prognosis is not yet satisfactory at the molecular level, and requires further improvements. Micro RNAs (miRNAs) have been recently reported as useful biomarkers in diseases including cancer. We performed a miRNA expression profiling study using peri...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-02, Vol.7 (2), p.e30049-e30049
Main Authors: Alshatwi, Ali A, Shafi, Gowhar, Hasan, Tarique N, Syed, Naveed Ahmed, Al-Hazzani, Amal A, Alsaif, Mohammed A, Alsaif, Abdulaziz A
Format: Article
Language:English
Subjects:
Adult
Age
Alleles
Angiogenesis
Base Sequence
Binding sites
Biology
Biomarkers
Blood
Breast cancer
Breast Neoplasms - blood
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cancer
Cancer research
Care and treatment
Case-Control Studies
Cell division
Development and progression
Diagnosis
Disease
Female
Food
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene sequencing
Genes
Genetic diversity
Genetic variance
Genetic Variation
Genomes
Genotyping
Genotyping Techniques
Health risk assessment
Health risks
Humans
Leukemia
Lung cancer
Medical diagnosis
Medicine
Menopause
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - blood
MicroRNAs - genetics
Middle Aged
miRNA
Mutation
Neoplasm Staging
Nutrition
Odds Ratio
Patients
Peripheral blood
Physiology
Population
Post-menopause
Postmenopausal women
Postmenopause - blood
Postmenopause - genetics
Prognosis
Ribonucleic acid
RNA
Stem cells
Womens health
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The technology available for cancer diagnosis and prognosis is not yet satisfactory at the molecular level, and requires further improvements. Micro RNAs (miRNAs) have been recently reported as useful biomarkers in diseases including cancer. We performed a miRNA expression profiling study using peripheral blood from breast cancer patients to detect and identify characteristic patterns. A total of 100 breast cancer patients and 89 healthy patients were recruited for miRNA genotyping and expression profiling. We found that hs-miR-196a2 in premenopausal patients, and hs-miR-499, hs-miR-146a and hs-miR-196a2 in postmenopausal patients, may discriminate breast cancer patients from healthy individuals. In addition, we found a significant association between two microRNA polymorphisms (hs-miR-196a2 and hs-miR-499) and breast cancer risk. However, no significant association between the hs-miR-146a gene and breast cancer risk was found. In summary, the study demonstrates that peripheral blood miRNAs and their expression and genotypic profiles can be developed as biomarkers for early diagnosis and prognosis of breast cancer.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0030049