Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification

Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-02, Vol.7 (2), p.e31068-e31068
Main Authors: Göransson, Jenny, Ke, Rongqin, Nong, Rachel Yuan, Howell, W Mathias, Karman, Anna, Grawé, Jan, Stenberg, Johan, Granberg, Malin, Elgh, Magnus, Herthnek, David, Wikström, Per, Jarvius, Jonas, Nilsson, Mats
Format: Article
Language:English
Subjects:
Alzheimer's disease
Amplification
Bacillus - genetics
Bacillus - metabolism
Biology
Biomarkers
Biosecurity
Bioterrorism
Chemistry
Cloning
Deoxyribonucleic acid
DNA
DNA - genetics
DNA Ligases - metabolism
DNA, Circular - analysis
E coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fluorescence
Fluorescent Dyes - pharmacology
Genetics
Hostages
Immunology
Laboratories
Medicine
Microscopy, Confocal - methods
Models, Genetic
Nucleic Acid Amplification Techniques
Oligonucleotide Probes
Oligonucleotides - genetics
Ovarian cancer
Pantoea - genetics
Pantoea - metabolism
Pathogens
Pathology
Proteins
Protocol
Spores
Target recognition
Technology application
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0031068