DPEP1 inhibits tumor cell invasiveness, enhances chemosensitivity and predicts clinical outcome in pancreatic ductal adenocarcinoma

• Published in: PloS one 2012-02, Vol.7 (2), p.e31507
• Main Authors: Zhang, Geng, Schetter, Aaron, He, Peijun, Funamizu, Naotake, Gaedcke, Jochen, Ghadimi, B Michael, Ried, Thomas, Hassan, Raffit, Yfantis, Harris G, Lee, Dong H, Lacy, Curtis, Maitra, Anirban, Hanna, Nader, Alexander, H Richard, Hussain, S Perwez
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Adult; Aged; Aged, 80 and over; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Biology; Breast cancer; Cancer; Cancer therapies; Carcinoma, Pancreatic Ductal - drug therapy; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - mortality; Carcinoma, Pancreatic Ductal - pathology; Cell adhesion & migration; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; Cell growth; Cell Line, Tumor; Chemotherapy; Chromosomes; Cohort Studies; Comparative analysis; Correlation coefficient; Correlation coefficients; Deoxycytidine - analogs & derivatives; Deoxycytidine - pharmacology; Deoxycytidine - therapeutic use; Dipeptidase; Dipeptidases - genetics; Dipeptidases - metabolism; DNA microarrays; Epidermal growth factor; Female; Gemcitabine; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene regulation; Genes; Genetic aspects; Genetic Association Studies; Genomics; GPI-Linked Proteins - genetics; GPI-Linked Proteins - metabolism; Humans; Invasiveness; Laboratories; Male; MAP Kinase Signaling System - drug effects; Maryland - epidemiology; Medical research; Medicine; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Middle Aged; Mortality; Neoplasm Invasiveness; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Oligonucleotide Array Sequence Analysis; Oxidative stress; Pancreatic cancer; Pancreatic Neoplasms - drug therapy; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - mortality; Pancreatic Neoplasms - pathology; Pathology; Patient outcomes; Patients; Prognosis; Proportional Hazards Models; Quality; Reproducibility of Results; Surgery; Tissues; Treatment Outcome; Tumor cells; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0031507

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers worldwide. To identify biologically relevant genes with prognostic and therapeutic significance in PDAC, we first performed the microarray gene-expression profiling in 45 matching pairs of tumor and adjacent non-tumor tissues from resected PDAC cases. We identified 36 genes that were associated with patient outcome and also differentially expressed in tumors as compared with adjacent non-tumor tissues in microarray analysis. Further evaluation in an independent validation cohort (N = 27) confirmed that DPEP1 (dipeptidase 1) expression was decreased (T:N ratio ∼0.1, P