Identification of cancer cell-line origins using fluorescence image-based phenomic screening

Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI's 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluor...

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Bibliographic Details
Published in:PloS one 2012-02, Vol.7 (2), p.e32096-e32096
Main Authors: Lee, Jun-Seok, Kim, Yun Kyung, Kim, Han Jo, Hajar, Siti, Tan, Yee Ling, Kang, Nam-Young, Ng, Shin Hui, Yoon, Chang No, Chang, Young-Tae
Format: Article
Language:English
Subjects:
Algorithms
Apoptosis
Biology
Boron Compounds - pharmacology
Cancer
Cell cycle
Cell Line, Tumor
Chemistry
Cluster Analysis
Colorectal cancer
Consortia
Fluorescence
Fluorescent Dyes - chemistry
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genetic aspects
Genomics
Genotype & phenotype
Humans
Kidney cancer
Kinetics
Library collections
Life sciences
Medical screening
Medicine
MicroRNAs
Microscopy, Fluorescence - methods
Organic Chemicals - pharmacology
Phenotype
Phenotyping
Rodents
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Summary:Universal phenotyping techniques that can discriminate among various states of biological systems have great potential. We applied 557 fluorescent library compounds to NCI's 60 human cancer cell-lines (NCI-60) to generate a systematic fluorescence phenotypic profiling data. By the kinetic fluorescence intensity analysis, we successfully discriminated the organ origin of all the 60 cell-lines.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0032096