Detailed mitochondrial phenotyping by high resolution metabolomics

Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spec...

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Bibliographic Details
Published in:PloS one 2012-03, Vol.7 (3), p.e33020-e33020
Main Authors: Roede, James R, Park, Youngja, Li, Shuzhao, Strobel, Frederick H, Jones, Dean P
Format: Article
Language:English
Subjects:
Alcohol
Alzheimer's disease
Alzheimers disease
Amino acids
Animals
Apoptosis
Biochemistry
Bioinformatics
Biology
Biomarkers
Chromatography
Critical care
Discriminant analysis
Fatty acids
Female
Gender differences
Genomes
High resolution
Ions
Liver
Male
Mass spectrometry
Medicine
Metabolic Networks and Pathways
Metabolic pathways
Metabolism
Metabolites
Metabolome
Metabolomics
Metabolomics - methods
Mice
Mice, Transgenic
Mitochondria
Mitochondria - genetics
Mitochondria - metabolism
Molecular biology
NMR
Nuclear magnetic resonance
Pathogenesis
Phenotype
Phenotyping
Physiological aspects
Physiology
Profiling
Proteins
Pyrimidines
Sex Factors
Statistical analysis
Statistical tests
Thioredoxins - genetics
Thioredoxins - metabolism
Urine
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Description
Summary:Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate statistical tests provided global metabolic information about mitochondria and showed that liver mitochondria possess a significant phenotype based on gender and genotype. The data also show that mitochondria contain a large number of unidentified chemicals.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0033020