Enteric infection with Citrobacter rodentium induces coagulative liver necrosis and hepatic inflammation prior to peak infection and colonic disease

• Published in: PloS one 2012-03, Vol.7 (3), p.e33099-e33099
• Main Authors: Raczynski, Arkadiusz R, Muthupalani, Sureshkumar, Schlieper, Katherine, Fox, James G, Tannenbaum, Steven R, Schauer, David B
• Format: Article
• Language: English
• Subjects: Analysis; Analysis of Variance; Animal models; Animals; Bacteria; Bioengineering; Biology; Cellular signal transduction; Chemokines - blood; Chronic infection; Citrobacter; Citrobacter rodentium; Colon; Colonic Diseases - microbiology; Colonization; Cytochrome; Cytokines; Cytokines - blood; Defects; Disease; Diseases; E coli; Edema; Engineering; Enzymes; Escherichia coli; Gangrene; Gastrointestinal diseases; Hepatitis; Hepatitis - microbiology; Homeostasis; House mouse; Immunoblotting; Immunohistochemistry; Infections; Inflammation; Inflammatory bowel disease; Injuries; Intestinal microflora; Intestine; Ischemia; Liver; Liver - microbiology; Liver - pathology; Medicine; Metabolism; Mice; Mice, Inbred C57BL; Microbiota; Microbiota (Symbiotic organisms); Necrosis; Necrosis - microbiology; Pathogenesis; Pathogens; Proteins; Rodents; Signal transduction; Signal Transduction - physiology; Statistics, Nonparametric; Stress; transaminase; Transaminases
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0033099

Acute and chronic forms of inflammation are known to affect liver responses and susceptibility to disease and injury. Furthermore, intestinal microbiota has been shown critical in mediating inflammatory host responses in various animal models. Using C. rodentium, a known enteric bacterial pathogen, we examined liver responses to gastrointestinal infection at various stages of disease pathogenesis. For the first time, to our knowledge, we show distinct liver pathology associated with enteric infection with C. rodentium in C57BL/6 mice, characterized by increased inflammation and hepatitis index scores as well as prominent periportal hepatocellular coagulative necrosis indicative of thrombotic ischemic injury in a subset of animals during the early course of C. rodentium pathogenesis. Histologic changes in the liver correlated with serum elevation of liver transaminases, systemic and liver resident cytokines, as well as signal transduction changes prior to peak bacterial colonization and colonic disease. C. rodentium infection in C57BL/6 mice provides a potentially useful model to study acute liver injury and inflammatory stress under conditions of gastrointestinal infection analogous to enteropathogenic E. coli infection in humans.