Analysis of IL12B gene variants in inflammatory bowel disease

• Published in: PloS one 2012-03, Vol.7 (3), p.e34349-e34349
• Main Authors: Glas, Jürgen, Seiderer, Julia, Wagner, Johanna, Olszak, Torsten, Fries, Christoph, Tillack, Cornelia, Friedrich, Matthias, Beigel, Florian, Stallhofer, Johannes, Steib, Christian, Wetzke, Martin, Göke, Burkhard, Ochsenkühn, Thomas, Diegelmann, Julia, Czamara, Darina, Brand, Stephan
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alleles; Binding; Biology; Carriers; Cholera; Colitis, Ulcerative - genetics; Collaboration; Crohn Disease - genetics; Crohn's disease; Crohns disease; Cytokines; Dentistry; Deoxyribonucleic acid; Disease prevention; Disease susceptibility; DNA; DNA binding proteins; Epigenetics; Epistasis; Female; Genes; Genetic aspects; Genetics; Genomes; Genomics; Haplotypes; Helper cells; HSF1 protein; Humans; IL12B protein; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Inflammatory Bowel Diseases - genetics; Interleukin 1; Interleukin 12; Interleukin 23; Interleukin-12 Subunit p40 - genetics; Intestine; Lymphocytes; Lymphocytes T; Male; Medical research; Medicine; Middle Aged; Oct-1 protein; Patients; Phenotype; Polymorphism, Single Nucleotide; Population; Psoriasis; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Stat4 protein; Studies; Transcription (Genetics); Transcription factors; Ulcerative colitis; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0034349

IL12B encodes the p40 subunit of IL-12, which is also part of IL-23. Recent genome-wide association studies identified IL12B and IL23R as susceptibility genes for inflammatory bowel disease (IBD). However, the phenotypic effects and potential gene-gene interactions of IL12B variants are largely unknown. We analyzed IL12B gene variants regarding association with Crohn's disease (CD) and ulcerative colitis (UC). Genomic DNA from 2196 individuals including 913 CD patients, 318 UC patients and 965 healthy, unrelated controls was analyzed for four SNPs in the IL12B gene region (rs3212227, rs17860508, rs10045431, rs6887695). Our analysis revealed an association of the IL12B SNP rs6887695 with susceptibility to IBD (p = 0.035; OR 1.15 [95% CI 1.01-1.31] including a trend for rs6887695 for association with CD (OR 1.41; [0.99-1.31], p = 0.066) and UC (OR 1.18 [0.97-1.43], p = 0.092). CD patients, who were homozygous C/C carriers of this SNP, had significantly more often non-stricturing, non-penetrating disease than carriers of the G allele (p = 6.8×10(-5); OR = 2.84, 95% CI 1.66-4.84), while C/C homozygous UC patients had less often extensive colitis than G allele carriers (p = 0.029; OR = 0.36, 95% CI 0.14-0.92). In silico analysis predicted stronger binding of the minor C allele of rs6887695 to the transcription factor RORα which is involved in Th17 differentiation. Differences regarding the binding to the major and minor allele sequence of rs6887695 were also predicted for the transcription factors HSF1, HSF2, MZF1 and Oct-1. Epistasis analysis revealed weak epistasis of the IL12B SNP rs6887695 with several SNPs (rs11889341, rs7574865, rs7568275, rs8179673, rs10181656, rs7582694) in the STAT4 gene which encodes the major IL-12 downstream transcription factor STAT4 (p