The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques

• Published in: PloS one 2012-04, Vol.7 (4), p.e34433-e34433
• Main Authors: Wang, Yufei, Whittall, Trevor, Rahman, Durdana, Bunnik, Evelien M, Vaughan, Robert, Schøller, Jørgen, Bergmeier, Lesley A, Montefiori, David, Singh, Mahavir, Schuitemaker, Hanneke, Lehner, Thomas
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptive Immunity - immunology; Animals; Antibodies; Antigens; Apolipoprotein B; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biology; CD20 antigen; CD27 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; CD40L protein; Cell activation; Cell interactions; Class switching; Cytidine Deaminase - metabolism; Deoxyribonucleic acid; Dextran; Diversification; DNA; E coli; Effector cells; Female; Heat shock proteins; Histocompatibility antigen HLA; HIV; HIV - immunology; HIV Infections - immunology; HIV Infections - metabolism; Homeostasis; Hsp70 protein; Human immunodeficiency virus; Humans; Immune response; Immunity; Immunity, Innate - immunology; Immunization; Immunoglobulin A; Immunoglobulin G; Immunoglobulins; Immunological memory; Immunology; Infections; Innate immunity; Interleukin 15; Interleukin 15 receptors; Interleukin-15 - metabolism; Kinases; Lymphocytes; Lymphocytes B; Lymphocytes T; Macaca mulatta; Medical research; Medicine; Memory cells; mRNA; Mutagenesis; Myeloid cells; Receptors, Interleukin-15; RNA; Simian Acquired Immunodeficiency Syndrome - immunology; Simian Acquired Immunodeficiency Syndrome - metabolism; Simian Immunodeficiency Virus - immunology; T cells; Vaccines
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0034433

The AID/APOBEC family (activation induced deaminase/apolipoprotein B mRNA editing cytokine deaminase) in B cells play important roles in adaptive and innate immunity. Whereas APOBEC3G has been studied in CD4+ T cells and myeloid cells its functional potential in B cells has received little attention. AID combines two critical functions of antibodies, class switching and affinity maturation and may serve as a functional surrogate of protection. These functions were studied following systemic immunization of rhesus macaques with recombinant HLA constructs, linked with HIV and SIV antigens and HSP70 to dextran. The results showed significant upregulation of AID in CD20+ B cells, APOBEC 3G in CD27+ memory B cells and CD4+ effector memory T cells. After immunization the upregulated APOBEC 3G and AID were directly correlated in B cells (p