The role of the Yap5 transcription factor in remodeling gene expression in response to Fe bioavailability

The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes...

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Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e37434
Main Authors: Pimentel, Catarina, Vicente, Cristina, Menezes, Regina Andrade, Caetano, Soraia, Carreto, Laura, Rodrigues-Pousada, Claudina
Format: Article
Language:English
Subjects:
Analysis
Arsenic
Baking yeast
Basic-Leucine Zipper Transcription Factors - genetics
Basic-Leucine Zipper Transcription Factors - physiology
Bioavailability
Biology
Biosynthesis
Cation Transport Proteins - biosynthesis
Deprivation
DNA microarrays
Drug resistance
Gene Expression
Gene Expression Regulation, Fungal - drug effects
Gene Expression Regulation, Fungal - physiology
Genes
Genetic aspects
Glutaredoxin
Glutaredoxins - biosynthesis
Homeostasis
Iron
Iron - metabolism
Iron - pharmacology
Iron Overload - physiopathology
Localization
Metabolism
Nuclear Localization Signals - physiology
Oxidative stress
Proteins
Respiration
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - biosynthesis
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Saccharomyces cerevisiae Proteins - physiology
Sulfur
Target recognition
Toxicity
Transcription factors
Transcription Factors - metabolism
Yeast
Yes-associated protein
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Summary:The budding yeast Saccharomyces cerevisiae has developed several mechanisms to avoid either the drastic consequences of iron deprivation or the toxic effects of iron excess. In this work, we analysed the global gene expression changes occurring in yeast cells undergoing iron overload. Several genes directly or indirectly involved in iron homeostasis showed altered expression and the relevance of these changes are discussed. Microarray analyses were also performed to identify new targets of the iron responsive factor Yap5. Besides the iron vacuolar transporter CCC1, Yap5 also controls the expression of glutaredoxin GRX4, previously known to be involved in the regulation of Aft1 nuclear localization. Consistently, we show that in the absence of Yap5 Aft1 nuclear exclusion is slightly impaired. These studies provide further evidence that cells control iron homeostasis by using multiple pathways.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0037434