BC4707 is a major facilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance

Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of th...

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Published in:PloS one 2012-05, Vol.7 (5), p.e36720-e36720
Main Authors: Simm, Roger, Vörös, Aniko, Ekman, Jaakko V, Sødring, Marianne, Nes, Ingerid, Kroeger, Jasmin K, Saidijam, Massoud, Bettaney, Kim E, Henderson, Peter J F, Salkinoja-Salonen, Mirja, Kolstø, Anne-Brit
Format: Article
Language:English
Subjects:
Activation
Analysis
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics
Bacillus anthracis
Bacillus cereus
Bacillus cereus - drug effects
Bacillus cereus - growth & development
Bacillus cereus - metabolism
Bacteria
Bacterial Proteins - physiology
Bile
Bile Acids and Salts - metabolism
Bile salts
Bioinformatics
Biological & chemical weapons
Biology
Biopesticides
Cell division
Deactivation
Drug resistance
Drug Resistance, Multiple
E coli
Efflux
Escherichia coli
Fluoroquinolones - pharmacology
Gene Silencing
Genes
Genetic aspects
Genomes
Genomics
Homeostasis
Inactivation
Laboratories
Metabolism
Metabolites
Microbial drug resistance
Molecular biology
Multidrug resistance
Multidrug resistant organisms
Norfloxacin
Oligonucleotide Array Sequence Analysis
Pathogens
Pharmaceuticals
Pharmacy
Physiological aspects
Protein Transport
Proteins
Salts
Stress, Physiological
Transcription
Transcription (Genetics)
Transcription, Genetic
Xenobiotics
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creator Simm, Roger
Vörös, Aniko
Ekman, Jaakko V
Sødring, Marianne
Nes, Ingerid
Kroeger, Jasmin K
Saidijam, Massoud
Bettaney, Kim E
Henderson, Peter J F
Salkinoja-Salonen, Mirja
Kolstø, Anne-Brit
description Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more specific, but modest role in the efflux of norfloxacin. In addition to this, transcriptional analyses showed that BC4707 is also expressed during growth of B. cereus under non-stressful conditions where it may have a role in the normal physiology of the bacteria. Altogether, the results indicate that bc4707, which is part of the core genome of the B. cereus group of bacteria, encodes a multidrug resistance efflux protein that is likely involved in maintaining intracellular homeostasis during growth of the bacteria.
doi_str_mv 10.1371/journal.pone.0036720
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subjects Activation
Analysis
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics
Bacillus anthracis
Bacillus cereus
Bacillus cereus - drug effects
Bacillus cereus - growth & development
Bacillus cereus - metabolism
Bacteria
Bacterial Proteins - physiology
Bile
Bile Acids and Salts - metabolism
Bile salts
Bioinformatics
Biological & chemical weapons
Biology
Biopesticides
Cell division
Deactivation
Drug resistance
Drug Resistance, Multiple
E coli
Efflux
Escherichia coli
Fluoroquinolones - pharmacology
Gene Silencing
Genes
Genetic aspects
Genomes
Genomics
Homeostasis
Inactivation
Laboratories
Metabolism
Metabolites
Microbial drug resistance
Molecular biology
Multidrug resistance
Multidrug resistant organisms
Norfloxacin
Oligonucleotide Array Sequence Analysis
Pathogens
Pharmaceuticals
Pharmacy
Physiological aspects
Protein Transport
Proteins
Salts
Stress, Physiological
Transcription
Transcription (Genetics)
Transcription, Genetic
Xenobiotics
title BC4707 is a major facilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance
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