Peripheral T cell cytokine responses for diagnosis of active tuberculosis

A test for diagnosis of active Tuberculosis (TB) from peripheral blood could tremendously improve clinical management of patients. Of 178 prospectively enrolled patients with possible TB, 60 patients were diagnosed with pulmonary and 27 patients with extrapulmonary TB. The frequencies of Mycobacteri...

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Published in:PloS one 2012-04, Vol.7 (4), p.e35290-e35290
Main Authors: Nemeth, Johannes, Winkler, Heide-Maria, Zwick, Ralph H, Müller, Catharina, Rumetshofer, Rudolf, Boeck, Lucas, Burghuber, Otto C, Winkler, Stefan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Antigens
Antigens, Bacterial
Bacterial Proteins
Biological response modifiers
CD4 antigen
CD4-Positive T-Lymphocytes - immunology
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
Critical care
Cytokines
Cytokines - immunology
Diagnostic software
Diagnostic systems
Drug therapy
Enzymes
Epidemiology
Female
Fever
Health aspects
Hospitals
Humans
Immunization
Infections
Infectious diseases
Interferon
Interferon-gamma
Interleukin-2
Interleukins
Internal medicine
Lymphocytes
Lymphocytes T
Male
Medical diagnosis
Medicine
Middle Aged
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Patients
Peripheral blood
T cell receptors
T cells
T-Lymphocytes - immunology
Tropical diseases
Tuberculin Test
Tuberculosis
Tuberculosis - diagnosis
Tumor necrosis factor-TNF
γ-Interferon
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cited_by cdi_FETCH-LOGICAL-c692t-eb36a401f91d7c8a733a8ffc33c2631431502ebc1eb2ccad89e9801e5c95dd583
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container_end_page e35290
container_issue 4
container_start_page e35290
container_title PloS one
container_volume 7
creator Nemeth, Johannes
Winkler, Heide-Maria
Zwick, Ralph H
Müller, Catharina
Rumetshofer, Rudolf
Boeck, Lucas
Burghuber, Otto C
Winkler, Stefan
description A test for diagnosis of active Tuberculosis (TB) from peripheral blood could tremendously improve clinical management of patients. Of 178 prospectively enrolled patients with possible TB, 60 patients were diagnosed with pulmonary and 27 patients with extrapulmonary TB. The frequencies of Mycobacterium tuberculosis (MTB) specific CD4(+) T cells and CD8(+) T cells producing cytokines were assessed using overnight stimulation with purified protein derivate (PPD) or early secretory antigenic target (ESAT)-6, respectively. Among patients with active TB, an increased type 1 cytokine profile consisting of mainly CD4(+) T cell derived interferon (IFN)-γ was detectable. Despite contributing to the cytokine profile as a whole, the independent diagnostic performance of one cytokine producing T cells as well as polyfunctional T cells was poor. IFN-γ/Interleukin(IL)-2 cytokine ratios discriminated best between active TB and other diseases. T cells producing one cytokine and polyfunctional T cells have a limited role in diagnosis of active TB. The significant shift from a "memory type" to an "effector type" cytokine profile may be useful for further development of a rapid immune-diagnostic tool for active TB.
doi_str_mv 10.1371/journal.pone.0035290
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source Publicly Available Content Database; PubMed Central
subjects Adolescent
Adult
Aged
Aged, 80 and over
Antigens
Antigens, Bacterial
Bacterial Proteins
Biological response modifiers
CD4 antigen
CD4-Positive T-Lymphocytes - immunology
CD8 antigen
CD8-Positive T-Lymphocytes - immunology
Critical care
Cytokines
Cytokines - immunology
Diagnostic software
Diagnostic systems
Drug therapy
Enzymes
Epidemiology
Female
Fever
Health aspects
Hospitals
Humans
Immunization
Infections
Infectious diseases
Interferon
Interferon-gamma
Interleukin-2
Interleukins
Internal medicine
Lymphocytes
Lymphocytes T
Male
Medical diagnosis
Medicine
Middle Aged
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Patients
Peripheral blood
T cell receptors
T cells
T-Lymphocytes - immunology
Tropical diseases
Tuberculin Test
Tuberculosis
Tuberculosis - diagnosis
Tumor necrosis factor-TNF
γ-Interferon
title Peripheral T cell cytokine responses for diagnosis of active tuberculosis
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