Antibodies for assessing circadian clock proteins in the rodent suprachiasmatic nucleus

Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label th...

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Bibliographic Details
Published in:PloS one 2012-04, Vol.7 (4), p.e35938-e35938
Main Authors: LeSauter, Joseph, Lambert, Christopher M, Robotham, Margaret R, Model, Zina, Silver, Rae, Weaver, David R
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies - immunology
Antigens
Arvicanthis niloticus
Biological clocks
Biology
BMAL1 protein
Brain
Circadian Clocks - immunology
Circadian rhythm
Circadian rhythms
CLOCK protein
CLOCK Proteins - immunology
Cloning
Cricetinae
Disruption
Gene expression
Hamsters
Immunocytochemistry
Immunoglobulins
Laboratory animals
Male
Mesocricetus
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Neurobiology
Neurosciences
Period 1 protein
Period 2 protein
Pets
Physiological aspects
Pregnancy
Proteins
Staining and Labeling
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - cytology
Suprachiasmatic Nucleus - metabolism
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Summary:Research on the mechanisms underlying circadian rhythmicity and the response of brain and body clocks to environmental and physiological challenges requires assessing levels of circadian clock proteins. Too often, however, it is difficult to acquire antibodies that specifically and reliably label these proteins. Many of these antibodies also lack appropriate validation. The goal of this project was to generate and characterize antibodies against several circadian clock proteins. We examined mice and hamsters at peak and trough times of clock protein expression in the suprachiasmatic nucleus (SCN). In addition, we confirmed specificity by testing the antibodies on mice with targeted disruption of the relevant genes. Our results identify antibodies against PER1, PER2, BMAL1 and CLOCK that are useful for assessing circadian clock proteins in the SCN by immunocytochemistry.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0035938