Deep and clear optical imaging of thick inhomogeneous samples

Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a...

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Bibliographic Details
Published in:PloS one 2012-04, Vol.7 (4), p.e35795-e35795
Main Authors: Jorand, Raphael, Le Corre, Gwénaële, Andilla, Jordi, Maandhui, Amina, Frongia, Céline, Lobjois, Valérie, Ducommun, Bernard, Lorenzo, Corinne
Format: Article
Language:English
Subjects:
Adaptive optics
Astronomical Sciences
Beads
Biological effects
Biology
Cell division
Emitters
Fluorescence
Focal plane
Image quality
Imaging, Three-Dimensional - methods
Inhomogeneity
Lasers
Light
Light microscopy
Lighting
Materials Science
Microscopy
Microscopy - instrumentation
Microscopy - methods
Optical Devices
Optics
Optics and Photonics - instrumentation
Optics and Photonics - methods
Sensors
Stars & galaxies
Three dimensional imaging
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Summary:Inhomogeneity in thick biological specimens results in poor imaging by light microscopy, which deteriorates as the focal plane moves deeper into the specimen. Here, we have combined selective plane illumination microscopy (SPIM) with wavefront sensor adaptive optics (wao). Our waoSPIM is based on a direct wavefront measure using a Hartmann-Shack wavefront sensor and fluorescent beads as point source emitters. We demonstrate the use of this waoSPIM method to correct distortions in three-dimensional biological imaging and to improve the quality of images from deep within thick inhomogeneous samples.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0035795