Genome and transcriptome analysis of the food-yeast Candida utilis

The industrially important food-yeast Candida utilis is a Crabtree effect-negative yeast used to produce valuable chemicals and recombinant proteins. In the present study, we conducted whole genome sequencing and phylogenetic analysis of C. utilis, which showed that this yeast diverged long before t...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e37226
Main Authors: Tomita, Yasuyuki, Ikeo, Kazuho, Tamakawa, Hideyuki, Gojobori, Takashi, Ikushima, Shigehito
Format: Article
Language:English
Subjects:
Accuracy
Alcohol dehydrogenase
Alcohols
Amino Acid Sequence
Analysis
Antisense RNA
Baking yeast
Base Sequence
Bioinformatics
Biology
Candida - genetics
Candida - metabolism
Candida albicans
Candida utilis
Chromosomes
Cladistic analysis
Deoxyribonucleic acid
DNA
DNA sequencing
Ethanol
Food industry
Gene expression
Gene Expression Profiling
Gene sequencing
Genes
Genetic aspects
Genetics
Genome, Fungal
Genomes
Genomics
Hexose
Hexose transporter
Kluyveromyces
Laboratories
Molecular Sequence Data
Phylogenetics
Phylogeny
Pichia
Proteins
Reagents
Recombinant proteins
Ribosomal DNA
Saccharomyces cerevisiae
Saccharomycetaceae
Transcription
Transcriptome
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The industrially important food-yeast Candida utilis is a Crabtree effect-negative yeast used to produce valuable chemicals and recombinant proteins. In the present study, we conducted whole genome sequencing and phylogenetic analysis of C. utilis, which showed that this yeast diverged long before the formation of the CUG and Saccharomyces/Kluyveromyces clades. In addition, we performed comparative genome and transcriptome analyses using next-generation sequencing, which resulted in the identification of genes important for characteristic phenotypes of C. utilis such as those involved in nitrate assimilation, in addition to the gene encoding the functional hexose transporter. We also found that an antisense transcript of the alcohol dehydrogenase gene, which in silico analysis did not predict to be a functional gene, was transcribed in the stationary-phase, suggesting a novel system of repression of ethanol production. These findings should facilitate the development of more sophisticated systems for the production of useful reagents using C. utilis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0037226