Identification, expression and target gene analyses of Micrornas in Spodoptera litura

MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence co...

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Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e37730-e37730
Main Authors: Rao, Zhongchen, He, Wenyin, Liu, Lin, Zheng, Sichun, Huang, Lihua, Feng, Qili
Format: Article
Language:English
Subjects:
Acids
Analysis
Animals
Apoptosis
Atherosclerosis
Base Sequence
Biology
Biomarkers
Biotechnology
Cluster Analysis
Computation
Computational Biology - methods
Computer applications
Conservation
Conserved sequence
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene regulation
Genes
Genetic aspects
Genomes
Hybridization
Hypoxia
Identification
Insects
Kinases
Ligands
Methods
MicroRNA
MicroRNAs
MicroRNAs - chemistry
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Molecular Sequence Data
Nucleic Acid Conformation
Organ Specificity - genetics
Phylogenetics
Phylogeny
Physiology
Polymerase chain reaction
Post-transcription
Protein structure
Secondary structure
Southern blotting
Spodoptera - classification
Spodoptera - genetics
Spodoptera litura
Target recognition
Tissues
Transcription (Genetics)
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Summary:MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-PCR was conducted to examine the expression of these miRNAs and stem-loop RT-PCR assay was performed to examine expression of 11 mature miRNAs (out of the 58 putative miRNA) that showed significant changes in different tissues and stages of the insect development. One hundred twenty eight possible target genes against the 11 miRNAs were predicted by using computational methods. Binding of one miRNA (sli-miR-928b) with the three possible target mRNAs was confirmed by Southern blotting, implying its possible function in regulation of the target genes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0037730