The ArcB leucine zipper domain is required for proper ArcB signaling

The Arc two-component system modulates the expression of numerous genes in response to respiratory growth conditions. This system comprises ArcA as the response regulator and ArcB as the sensor kinase. ArcB is a tripartite histidine kinase whose activity is regulated by the oxidation of two cytosol-...

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Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e38187
Main Authors: Nuñez Oreza, Luis Alberto, Alvarez, Adrián F, Arias-Olguín, Imilla I, Torres Larios, Alfredo, Georgellis, Dimitris
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis
ArcB protein
Automation
Biology
Chemical bonds
Coils
Cysteine
Cytosol
DNA binding proteins
E coli
Escherichia coli
Escherichia coli - enzymology
Escherichia coli Proteins - chemistry
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene expression
Growth conditions
Histidine
Histidine kinase
Kinases
Laboratories
Leucine
Leucine zipper proteins
Leucine Zippers
Membrane Proteins - chemistry
Membrane Proteins - genetics
Membrane Proteins - metabolism
Metabolism
Metabolites
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Oxidation
Phosphoric Monoester Hydrolases - metabolism
Protein Kinases - chemistry
Protein Kinases - genetics
Protein Kinases - metabolism
Proteins
Residues
Sensors
Sequence Analysis
Signaling
Transmitters
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Summary:The Arc two-component system modulates the expression of numerous genes in response to respiratory growth conditions. This system comprises ArcA as the response regulator and ArcB as the sensor kinase. ArcB is a tripartite histidine kinase whose activity is regulated by the oxidation of two cytosol-located redox-active cysteine residues that participate in intermolecular disulfide bond formation. Here, we report that the ArcB protein segment covering residues 70-121, fulfills the molecular characteristics of a leucine zipper containing coiled coil structure. Also, mutational analyses of this segment reveal three different phenotypical effects to be distributed along the coiled coil structure of ArcB, demonstrating that this motif is essential for proper ArcB signaling.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0038187