Generation of induced pluripotent stem cells from the prairie vole

The vast majority of animals mate more or less promiscuously. A few mammals, including humans, utilize more restrained mating strategies that entail a longer term affiliation with a single mating partner. Such pair bonding mating strategies have been resistant to genetic analysis because of a lack o...

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Bibliographic Details
Published in:PloS one 2012-05, Vol.7 (5), p.e38119-e38119
Main Authors: Manoli, Devanand S, Subramanyam, Deepa, Carey, Catriona, Sudin, Erik, Van Westerhuyzen, Julie A, Bales, Karen L, Blelloch, Robert, Shah, Nirao M
Format: Article
Language:English
Subjects:
Analysis
Animal behavior
Animal reproduction
Animals
Arvicolinae - metabolism
Biology
Cell culture
Cell Culture Techniques
Cell Differentiation
Cell Line
Chemical bonds
Embryos
Fibroblasts
Fibroblasts - cytology
Genetic analysis
Genetic engineering
Genetically modified organisms
Genetics
Homologous Recombination
Homology
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mating
Microtus ochrogaster
Morphology
Muscular dystrophy
Mutation
Neural circuitry
Neurobiology
Pair bond
Personal relationships
Phosphatase
Pluripotency
Psychiatry
Psychosis
Rodents
Schizophrenia
Small mammals
Social behavior
Stem cell transplantation
Stem cells
Tissue culture
Transgenes
Urology
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Summary:The vast majority of animals mate more or less promiscuously. A few mammals, including humans, utilize more restrained mating strategies that entail a longer term affiliation with a single mating partner. Such pair bonding mating strategies have been resistant to genetic analysis because of a lack of suitable model organisms. Prairie voles are small mouse-like rodents that form enduring pair bonds in the wild as well as in the laboratory, and consequently they have been used widely to study social bonding behavior. The lack of targeted genetic approaches in this species however has restricted the study of the molecular and neural circuit basis of pair bonds. As a first step in rendering the prairie vole amenable to reverse genetics, we have generated induced pluripotent stem cell (IPSC) lines from prairie vole fibroblasts using retroviral transduction of reprogramming factors. These IPSC lines display the cellular and molecular hallmarks of IPSC cells from other organisms, including mice and humans. Moreover, the prairie vole IPSC lines have pluripotent differentiation potential since they can give rise to all three germ layers in tissue culture and in vivo. These IPSC lines can now be used to develop conditions that facilitate homologous recombination and eventually the generation of prairie voles bearing targeted genetic modifications to study the molecular and neural basis of pair bond formation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0038119