Improvement of pest resistance in transgenic tobacco plants expressing dsRNA of an insect-associated gene EcR

• Published in: PloS one 2012-06, Vol.7 (6), p.e38572-e38572
• Main Authors: Zhu, Jin-Qi, Liu, Shumin, Ma, Yao, Zhang, Jia-Qi, Qi, Hai-Sheng, Wei, Zhao-Jun, Yao, Qiong, Zhang, Wen-Qing, Li, Sheng
• Format: Article
• Language: English
• Subjects: Agriculture; Agrochemicals; Animals; Biology; Biotechnology; Brugia malayi; Caenorhabditis elegans; Chemical pest control; Cotton; Crops; Disease Resistance - genetics; Double-stranded RNA; Ecdysone; Evolutionary biology; Gene expression; Gene sequencing; Genes; Genetic engineering; Genetically modified plants; Genomes; Genomics; Helicoverpa armigera; Host-Parasite Interactions - genetics; Insect Proteins - genetics; Insecticide resistance; Insecticides; Insects; Laboratories; Larva - genetics; Larva - growth & development; Larva - physiology; Larvae; Leaves; Lethality; Life sciences; Molting; Moths - genetics; Moths - growth & development; Moths - physiology; mRNA; Nematodes; Nicotiana - genetics; Nicotiana - growth & development; Nicotiana - parasitology; Pest control; Pest resistance; Pesticides; Pests; Physiology; Plant Diseases - genetics; Plant Diseases - parasitology; Plants, Genetically Modified; Receptors, Steroid - genetics; Ribonucleic acid; RNA; RNA, Double-Stranded - genetics; Signal transduction; Spodoptera exigua; Studies; Tobacco; Toxins; Transgenic plants
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0038572

The adoption of pest-resistant transgenic plants to reduce yield loss and pesticide utilization has been successful in the past three decades. Recently, transgenic plant expressing double-stranded RNA (dsRNA) targeting pest genes emerges as a promising strategy for improving pest resistance in crops. The steroid hormone, 20-hydroxyecdysone (20E), predominately controls insect molting via its nuclear receptor complex, EcR-USP. Here we report that pest resistance is improved in transgenic tobacco plants expressing dsRNA of EcR from the cotton bollworm, Helicoverpa armigera, a serious lepidopteran pest for a variety of crops. When H. armigera larvae were fed with the whole transgenic tobacco plants expressing EcR dsRNA, resistance to H. armigera was significantly improved in transgenic plants. Meanwhile, when H. armigera larvae were fed with leaves of transgenic tobacco plants expressing EcR dsRNA, its EcR mRNA level was dramatically decreased causing molting defects and larval lethality. In addition, the transgenic tobacco plants expressing H. armigera EcR dsRNA were also resistant to another lepidopteran pest, the beet armyworm, Spodoptera exigua, due to the high similarity in the nucleotide sequences of their EcR genes. This study provides additional evidence that transgenic plant expressing dsRNA targeting insect-associated genes is able to improve pest resistance.