Characterization of human coronavirus etiology in Chinese adults with acute upper respiratory tract infection by real-time RT-PCR assays

In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by mol...

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Bibliographic Details
Published in:PloS one 2012-06, Vol.7 (6), p.e38638-e38638
Main Authors: Lu, Roujian, Yu, Xiaoyan, Wang, Wen, Duan, Xijie, Zhang, Linglin, Zhou, Weimin, Xu, Jin, Xu, Lingjie, Hu, Qin, Lu, Jianxin, Ruan, Li, Wang, Zhong, Tan, Wenjie
Format: Article
Language:English
Subjects:
Acute Disease
Adults
Base Sequence
Biology
China - epidemiology
Consent
Coronaviridae
Coronavirus - genetics
Coronavirus - isolation & purification
Coronaviruses
Development and progression
Disease control
Disease prevention
DNA Primers
Education
Ethics
Etiology
Etiology (Medicine)
Hospitals
Humans
Infections
Influenza
Medical laboratories
Medicine
Nasopharynx - virology
Parainfluenza
Pathogens
Patients
Pneumonia
Polymerase chain reaction
Population Surveillance
Real time
Real-Time Polymerase Chain Reaction - methods
Respiratory diseases
Respiratory syncytial virus
Respiratory tract
Respiratory tract diseases
Respiratory tract infection
Respiratory Tract Infections - virology
Rhinovirus
Severe acute respiratory syndrome
Virology
Viruses
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Summary:In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by molecular detection with excellent sensitivity. In this study, we detected 4 non-SARS related HCoV species by real-time RT-PCR in 981 nasopharyngeal swabs collected from March 2009 to February 2011. All specimens were also tested for the presence of other common respiratory viruses and newly identified viruses, human metapneumovirus (hMPV) and human bocavirus (HBoV). 157 of the 981 (16.0%) nasopharyngeal swabs were positive for HCoVs. The species detected were 229E (96 cases, 9.8%), OC43 (42 cases, 4.3%), HKU1 (16 cases, 1.6%) and NL63 (11 cases, 1.1%). HCoV-229E was circulated in 21 of the 24 months of surveillance. The detection rates for both OC43 and NL63 were showed significantly year-to-year variation between 2009/10 and 2010/11, respectively (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0038638