Maturation of the mitochondrial redox response to profound asphyxia in fetal sheep

Fetal susceptibility to hypoxic brain injury increases over the last third of gestation. This study examined the hypothesis that this is associated with impaired mitochondrial adaptation, as measured by more rapid oxidation of cytochrome oxidase (CytOx) during profound asphyxia. Chronically instrume...

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Bibliographic Details
Published in:PloS one 2012-06, Vol.7 (6), p.e39273-e39273
Main Authors: Drury, Paul P, Bennet, Laura, Booth, Lindsea C, Davidson, Joanne O, Wassink, Guido, Gunn, Alistair J
Format: Article
Language:English
Subjects:
Animals
Asphyxia
Asphyxia - metabolism
Biology
Blood
Brain
Brain injuries
Brain injury
Cerebral blood flow
Cortex
Cytochrome
Cytotoxicity
Edema
EEG
Electroencephalography
Fetuses
Gestation
Gestational age
Head injuries
Hemoglobin
Hemoglobins
Hypotension
Hypotheses
Hypoxia
I.R. radiation
Impedance
Infrared spectra
Infrared spectroscopy
Ischemia
Lasers
Maturation
Medicine
Melatonin
Metabolism
Metabolites
Mitochondria
Mitochondria - metabolism
Musculoskeletal system
Near infrared radiation
Near infrared spectroscopy
Neurosciences
Occlusion
Optical properties
Ovis aries
Oxidases
Oxidation
Oxidation-Reduction
Oxidative metabolism
Oxygenation
Permeability
Physiological aspects
Physiology
Pregnancy
Redox properties
Sheep
Sheep - embryology
Spectroscopy, Near-Infrared
Spectrum analysis
Traumatic brain injury
Umbilical cord
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Summary:Fetal susceptibility to hypoxic brain injury increases over the last third of gestation. This study examined the hypothesis that this is associated with impaired mitochondrial adaptation, as measured by more rapid oxidation of cytochrome oxidase (CytOx) during profound asphyxia. Chronically instrumented fetal sheep at 0.6, 0.7, and 0.85 gestation were subjected to either 30 min (0.6 gestational age (ga), n = 6), 25 min (0.7 ga, n = 27) or 15 min (0.85 ga, n = 17) of complete umbilical cord occlusion. Fetal EEG, cerebral impedance (to measure brain swelling) and near-infrared spectroscopy-derived intra-cerebral oxygenation (ΔHb = HbO(2) - Hb), total hemoglobin (THb) and CytOx redox state were monitored continuously. Occlusion was associated with profound, rapid fall in ΔHb in all groups to a plateau from 6 min, greatest at 0.85 ga compared to 0.6 and 0.7 ga (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0039273