Using GFP video to track 3D movement and conditional gene expression in free-moving flies

In vivo imaging and quantification of fluorescent reporter molecules is increasingly useful in biomedical research. For example, tracking animal movement in 3D with simultaneous quantification of fluorescent transgenic reporters allows for correlations between behavior, aging and gene expression. Ho...

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Published in:PloS one 2012-07, Vol.7 (7), p.e40506-e40506
Main Authors: Ardekani, Reza, Huang, Yichuan Michelle, Sancheti, Prathamesh, Stanciauskas, Ramunas, Tavaré, Simon, Tower, John
Format: Article
Language:English
Subjects:
Aging
Animal experimentation
Animals
Animals, Genetically Modified
Automation
Behavior
Biology
Biomedical materials
Cameras
Computer Science
Diagnostic Imaging - methods
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Fluorescence
Gene expression
Gene Expression - genetics
Gene Expression - physiology
Genes
Genetic aspects
Genetic engineering
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Imaging, Three-Dimensional - methods
Insects
Journalists
Light
Neurosciences
Proteins
Retina - metabolism
Software
Studies
Three dimensional motion
Tissues
Tracking
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description In vivo imaging and quantification of fluorescent reporter molecules is increasingly useful in biomedical research. For example, tracking animal movement in 3D with simultaneous quantification of fluorescent transgenic reporters allows for correlations between behavior, aging and gene expression. However implementation has been hindered in the past by the complexity of operating the systems. We report significant technical improvements and user-friendly software (called FluoreScore) that enables tracking of 3D movement and the dynamics of gene expression in adult Drosophila, using two cameras and recorded GFP videos. Expression of a transgenic construct encoding eGFP was induced in free-moving adult flies using the Gene-Switch system and RU486 drug feeding. The time course of induction of eGFP expression was readily quantified from internal tissues including central nervous tissue. FluoreScore should facilitate a variety of future studies involving quantification of movement behaviors and fluorescent molecules in free-moving animals.
doi_str_mv 10.1371/journal.pone.0040506
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subjects Aging
Animal experimentation
Animals
Animals, Genetically Modified
Automation
Behavior
Biology
Biomedical materials
Cameras
Computer Science
Diagnostic Imaging - methods
Drosophila
Drosophila - genetics
Drosophila - metabolism
Drosophila melanogaster
Fluorescence
Gene expression
Gene Expression - genetics
Gene Expression - physiology
Genes
Genetic aspects
Genetic engineering
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Imaging, Three-Dimensional - methods
Insects
Journalists
Light
Neurosciences
Proteins
Retina - metabolism
Software
Studies
Three dimensional motion
Tissues
Tracking
title Using GFP video to track 3D movement and conditional gene expression in free-moving flies
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