StarD7 knockdown modulates ABCG2 expression, cell migration, proliferation, and differentiation of human choriocarcinoma JEG-3 cells

StAR-related lipid transfer domain containing 7 (StarD7) is a member of the START-domain protein family whose function still remains unclear. Our data from an explorative microarray assay performed with mRNAs from StarD7 siRNA-transfected JEG-3 cells indicated that ABCG2 (ATP-binding cassette sub-fa...

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Bibliographic Details
Published in:PloS one 2012-08, Vol.7 (8), p.e44152-e44152
Main Authors: Flores-Martín, Jésica, Rena, Viviana, Márquez, Sebastián, Panzetta-Dutari, Graciela M, Genti-Raimondi, Susana
Format: Article
Language:English
Subjects:
ATP Binding Cassette Transporter, Sub-Family G, Member 2
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - metabolism
Biology
Biomarkers - metabolism
Bromodeoxyuridine
Cancer
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell culture
Cell Differentiation - genetics
Cell Line, Tumor
Cell migration
Cell Movement - genetics
Cell Proliferation
Choriocarcinoma
Choriocarcinoma - genetics
Choriocarcinoma - pathology
Chorionic Gonadotropin, beta Subunit, Human - genetics
Chorionic Gonadotropin, beta Subunit, Human - metabolism
Desmosomes
Differentiation
Experiments
Gene expression
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Gene Products, env - genetics
Gene Products, env - metabolism
Gene Silencing
Giant Cells - metabolism
Humans
Kinases
Lipids
Localization
Medicine
Metabolism
mRNA
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Phospholipids
Phospholipids - biosynthesis
Pituitary (anterior)
Pregnancy Proteins - genetics
Pregnancy Proteins - metabolism
Protein biosynthesis
Protein expression
Protein synthesis
Proteins
Reduction
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Small Interfering - metabolism
Rodents
siRNA
Syncytin
Thymidine
Tumors
Up-Regulation - genetics
Wound healing
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Description
Summary:StAR-related lipid transfer domain containing 7 (StarD7) is a member of the START-domain protein family whose function still remains unclear. Our data from an explorative microarray assay performed with mRNAs from StarD7 siRNA-transfected JEG-3 cells indicated that ABCG2 (ATP-binding cassette sub-family G member 2) was one of the most abundantly downregulated mRNAs. Here, we have confirmed that knocking down StarD7 mRNA lead to a decrease in the xenobiotic/lipid transporter ABCG2 at both the mRNA and protein levels (-26.4% and -41%, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0044152