Over-expression of monoacylglycerol lipase (MGL) in small intestine alters endocannabinoid levels and whole body energy balance, resulting in obesity

• Published in: PloS one 2012-08, Vol.7 (8), p.e43962-e43962
• Main Authors: Chon, Su-Hyoun, Douglass, John D, Zhou, Yin Xiu, Malik, Nashmia, Dixon, Joseph L, Brinker, Anita, Quadro, Loredana, Storch, Judith
• Format: Article
• Language: English
• Subjects: Adipose tissue; Adiposity - physiology; Agouti-Related Protein - metabolism; Animals; Appetite - physiology; Arachidonic Acids - metabolism; Basal Metabolism - physiology; Biology; Body fat; Body mass; Body weight; Body weight gain; Brain - metabolism; Cannabinoids; Diabetes; Diet; Eating - physiology; Endocannabinoids; Endocannabinoids - metabolism; Energy; Energy balance; Energy expenditure; Energy Metabolism - physiology; Enzymes; Fatty acids; Food intake; Gene expression; Genetic aspects; Genomes; Glycerides - metabolism; Glycerol; Insulin resistance; Intestine, Small - metabolism; Lean body mass; Lipase; Lipids; Medicine; Metabolic rate; Metabolism; Mice; Mice, Transgenic; Monoacylglycerol Lipases - genetics; Monoacylglycerol Lipases - metabolism; Mucosa; Neuropeptide Y - metabolism; Obesity; Obesity - genetics; Obesity - metabolism; Overexpression; Phenotypes; Physiological aspects; Polyunsaturated Alkamides - metabolism; Pro-Opiomelanocortin - metabolism; Proteins; Receptor, Cannabinoid, CB1 - metabolism; Risk factors; Rodents; Small intestine; Transgenic animals; Transgenic mice; Triglycerides; Triglycerides - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0043962

The function of small intestinal monoacylglycerol lipase (MGL) is unknown. Its expression in this tissue is surprising because one of the primary functions of the small intestine is to convert diet-derived MGs to triacylglycerol (TG), and not to degrade them. To elucidate the function of intestinal MGL, we generated transgenic mice that over-express MGL specifically in small intestine (iMGL mice). After only 3 weeks of high fat feeding, iMGL mice showed an obese phenotype; body weight gain and body fat mass were markedly higher in iMGL mice, along with increased hepatic and plasma TG levels compared to wild type littermates. The iMGL mice were hyperphagic and displayed reduced energy expenditure despite unchanged lean body mass, suggesting that the increased adiposity was due to both increased caloric intake and systemic effects resulting in a hypometabolic rate. The presence of the transgene resulted in lower levels of most MG species in intestinal mucosa, including the endocannabinoid 2-arachidonoyl glycerol (2-AG). The results therefore suggest a role for intestinal MGL, and intestinal 2-AG and perhaps other MG species, in whole body energy balance via regulation of food intake as well as metabolic rate.