Comparative analysis of single-species and polybacterial wound biofilms using a quantitative, in vivo, rabbit ear model

The recent literature suggests that chronic wound biofilms often consist of multiple bacterial species. However, without appropriate in vivo, polybacterial biofilm models, our understanding of these complex infections remains limited. We evaluate and compare the effect of single- and mixed-species b...

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Bibliographic Details
Published in:PloS one 2012-08, Vol.7 (8), p.e42897-e42897
Main Authors: Seth, Akhil K, Geringer, Matthew R, Hong, Seok J, Leung, Kai P, Galiano, Robert D, Mustoe, Thomas A
Format: Article
Language:English
Subjects:
Animal models
Animals
Antibiotics
Bacteria
Bacterial infections
Biofilms
Biology
Cell Line
Comparative analysis
Cytokines - metabolism
Deficient mutant
Ear
Ear - pathology
Electron microscopy
Extracellular matrix
Female
Healing
Health aspects
IL-1β
In vivo methods and tests
Infection
Infections
Inflammation
Media (selective)
Medicine
Mice
Microscopy, Electron, Scanning - methods
Pathogenicity
Pathogens
Plastic surgery
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - metabolism
Pseudomonas Infections - microbiology
Rabbits
RNA, Messenger - metabolism
Scanning electron microscopy
Selective media
Skin
Species
Staphylococcus
Staphylococcus aureus
Staphylococcus aureus - metabolism
Staphylococcus infections
Studies
Time Factors
Tumor necrosis factor-α
Virulence
Wound care
Wound Healing
Wound Infection - microbiology
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Summary:The recent literature suggests that chronic wound biofilms often consist of multiple bacterial species. However, without appropriate in vivo, polybacterial biofilm models, our understanding of these complex infections remains limited. We evaluate and compare the effect of single- and mixed-species biofilm infections on host wound healing dynamics using a quantitative, in vivo, rabbit ear model. Six-mm dermal punch wounds in New Zealand rabbit ears were inoculated with Staphylococcus aureus strain UAMS-1, Pseudomonas aeruginosa strain PAO1, or both, totaling 10/6 colony-forming units/wound. Bacterial proliferation and maintenance in vivo were done using procedures from our previously published model. Wounds were harvested for histological measurement of wound healing, viable bacterial counts using selective media, or inflammatory cytokine (IL-1β, TNF-α) expression via quantitative reverse-transcription PCR. Biofilm structure was studied using scanning electron microscopy (SEM). For comparison, biofilm deficient mutant UAMS-929 replaced strain UAMS-1 in some mixed-species infections. Bacterial counts verified the presence of both strains UAMS-1 and PAO1 in polybacterial wounds. Over time, strain PAO1 became predominant (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0042897