Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis

Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain re...

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Bibliographic Details
Published in:PloS one 2012-09, Vol.7 (9), p.e44287
Main Authors: Nogues, Claude, Leh, Hervé, Lautru, Joseph, Delelis, Olivier, Buckle, Malcolm
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Analytical chemistry
Antifouling
Antifouling substances
Binding
Biofouling - prevention & control
Biology
Biomolecules
Biosensors
Biotechnology
Chemical properties
Deoxyribonucleic acid
Detection equipment
DNA
DNA - metabolism
Hybridization
Immobilized Nucleic Acids - metabolism
Integrases - metabolism
Kinetics
Life Sciences
Ligands
Macromolecules
Models, Molecular
Monolayers
Physical chemistry
Protein Binding
Proteins
Quantitative analysis
Retroviridae - metabolism
Self assembly
Self-assembled monolayers
Surface plasmon resonance
Surface Plasmon Resonance - methods
Surface Properties
Time Factors
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Summary:Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0044287