Hpz1 modulates the G1-S transition in fission yeast

Here we characterize a novel protein in S. pombe. It has a high degree of homology with the Zn-finger domain of the human Poly(ADP-ribose) polymerase (PARP). Surprisingly, the gene for this protein is, in many fungi, fused with and in the same reading frame as that encoding Rad3, the homologue of th...

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Bibliographic Details
Published in:PloS one 2012-09, Vol.7 (9), p.e44539-e44539
Main Authors: Bøe, Cathrine A, Knutsen, Jon Halvor J, Boye, Erik, Grallert, Beáta
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Amino Acid Sequence
Bioinformatics
Biology
Cancer
Cell cycle
Cell Nucleus - metabolism
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA damage
DNA replication
Fungi
G1 Phase
Gene expression
Genomes
Homology
Humans
Hydroxyurea
Indexing in process
Ionizing radiation
Kinases
Localization
Medical research
Molecular Sequence Data
Monosaccharides
Nuclei
Poly(ADP-ribose)
Poly(ADP-ribose) polymerase
Proteins
Replication forks
Ribose
S Phase
Schizosaccharomyces - cytology
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - chemistry
Schizosaccharomyces pombe Proteins - metabolism
Schizosaccharomyces pombe Proteins - physiology
Sequence Homology, Amino Acid
U.V. radiation
Ultraviolet radiation
Yeast
Zinc
Zinc compounds
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Summary:Here we characterize a novel protein in S. pombe. It has a high degree of homology with the Zn-finger domain of the human Poly(ADP-ribose) polymerase (PARP). Surprisingly, the gene for this protein is, in many fungi, fused with and in the same reading frame as that encoding Rad3, the homologue of the human ATR checkpoint protein. We name the protein Hpz1 (Homologue of PARP-type Zn-finger). Hpz1 does not possess PARP activity, but is important for resistance to ultraviolet light in the G1 phase and to treatment with hydroxyurea, a drug that arrests DNA replication forks in the S phase. However, we find no evidence of a checkpoint function of Hpz1. Furthermore, absence of Hpz1 results in an advancement of S-phase entry after a G1 arrest as well as earlier recovery from a hydroxyurea block. The hpz1 gene is expressed mainly in the G1 phase and Hpz1 is localized to the nucleus. We conclude that Hpz1 regulates the initiation of the S phase and may cooperate with Rad3 in this function.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0044539