Involvement of the tubular ClC-type exchanger ClC-5 in glomeruli of human proteinuric nephropathies

Glomerular protein handling mechanisms have received much attention in studies of nephrotic syndrome. Histopathological findings in renal biopsies from severely proteinuric patients support the likelihood of protein endocytosis by podocytes. ClC-5 is involved in the endocytosis of albumin in the pro...

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Bibliographic Details
Published in:PloS one 2012-09, Vol.7 (9), p.e45605-e45605
Main Authors: Ceol, Monica, Tiralongo, Emilia, Baelde, Hans J, Vianello, Daniela, Betto, Giovanni, Marangelli, Annunziata, Bonfante, Luciana, Valente, Marialuisa, Della Barbera, Mila, D'Angelo, Angela, Anglani, Franca, Del Prete, Dorella
Format: Article
Language:English
Subjects:
Albumin
Biology
Biopsy
Care and treatment
Chloride Channels - genetics
Chloride Channels - metabolism
Chloride Channels - physiology
Development and progression
Diabetes mellitus
Diagnosis
Endocytosis
Feet
Gene expression
Genetic aspects
Humans
IgA nephropathy
Immunoglobulin A
Immunohistochemistry
Kidney diseases
Kidney Diseases - metabolism
Kidney Glomerulus - metabolism
Kidney transplantation
Kidney Tubules - metabolism
Kidneys
Laboratories
Lasers
Localization
Medicine
Molecular biology
Morphology
Morphometry
mRNA
Nephrectomy
Nephrology
Nephrotic syndrome
Pathology
Patients
Physiological aspects
Proteins
Proteinuria
Proteinuria - metabolism
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
Rodents
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Summary:Glomerular protein handling mechanisms have received much attention in studies of nephrotic syndrome. Histopathological findings in renal biopsies from severely proteinuric patients support the likelihood of protein endocytosis by podocytes. ClC-5 is involved in the endocytosis of albumin in the proximal tubule. To investigate whether ClC-5 is expressed in the glomerular compartment and whether it has a role in proteinuric nephropathies. ClC-5 expression was studied using Real-time PCR in manually- and laser-microdissected biopsies from patients with type 2 diabetes (n 37) and IgA nephropathy (n 10); in biopsies of membranous glomerulopathy (MG) (n 14) immunohistochemistry for ClC-5 (with morphometric analysis) and for WT1 was done. cortical tissue (n 23) obtained from unaffected parts of tumor-related nephrectomy specimens. ClC-5 was expressed at glomerular level in all biopsies. Glomerular ClC-5 levels were significantly higher in diabetic nephropaty and MG at both mRNA and protein level (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0045605