Zfp423 promotes adipogenic differentiation of bovine stromal vascular cells

• Published in: PloS one 2012-10, Vol.7 (10), p.e47496-e47496
• Main Authors: Huang, Yan, Das, Arun Kr, Yang, Qi-Yuan, Zhu, Mei-Jun, Du, Min
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipogenesis; Adipogenesis - physiology; Agriculture; Animal sciences; Animals; Azo Compounds; Beef; Beef cattle; Biology; Blotting, Western; Bone morphogenetic proteins; Bovidae; Cattle; CCAAT-Enhancer-Binding Protein-alpha - metabolism; CCAAT/enhancer-binding protein; Cell Differentiation - physiology; Cells (biology); Cloning; Deoxyribonucleic acid; Developmental biology; Differentiation; DNA; DNA binding proteins; DNA methylation; DNA Primers - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Feeds; Fibroblasts; Gene expression; Genomics; Growth factors; Insulin resistance; Kinases; Linear Models; Meat; Medicine; Methylation; Mice; Muscle, Skeletal - cytology; Muscles; Musculoskeletal system; Overexpression; Palatability; Plasmids - genetics; PPAR gamma - metabolism; Protein binding; Proteins; Real-Time Polymerase Chain Reaction; Receptor, Platelet-Derived Growth Factor alpha - metabolism; Stem cells; Stromal Cells - physiology; Surface markers; Transcription Factors - genetics; Transcription Factors - metabolism; Transfection; Transforming growth factor; Transforming Growth Factor beta - metabolism; Transforming growth factors; Type 2 diabetes; Veterinary Science; Weaning; Zinc; Zinc finger proteins; Zoology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0047496

Intramuscular fat or marbling is critical for the palatability of beef. In mice, very recent studies show that adipocytes and fibroblasts share a common pool of progenitor cells, with Zinc finger protein 423 (Zfp423) as a key initiator of adipogenic differentiation. To evaluate the role of Zfp423 in intramuscular adipogenesis and marbling in beef cattle, we sampled beef muscle for separation of stromal vascular cells. These cells were immortalized with pCI neo-hEST2 and individual clones were selected by G418. A total of 288 clones (3×96 well plates) were isolated and induced to adipogenesis. The presence of adipocytes was assessed by Oil-Red-O staining. Three clones with high and low adipogenic potential respectively were selected for further analyses. In addition, fibro/adipogenic progenitor cells were selected using a surface marker, platelet derived growth factor receptor (PDGFR) α. The expression of Zfp423 was much higher (307.4±61.9%, P