The τCstF-64 polyadenylation protein controls genome expression in testis

The τCstF-64 polyadenylation protein (gene symbol Cstf2t) is a testis-expressed orthologue of CstF-64. Mice in which Cstf2t was knocked out had a phenotype that was only detected in meiotic and postmeiotic male germ cells, giving us the opportunity to examine CstF-64 function in an isolated developm...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e48373-e48373
Main Authors: Li, Wencheng, Yeh, Hsiang-Jui, Shankarling, Ganesh S, Ji, Zhe, Tian, Bin, MacDonald, Clinton C
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological evolution
Biology
Cancer
Cleavage Stimulation Factor - genetics
Cleavage Stimulation Factor - metabolism
Gene expression
Genes
Genomes
Genomics
Germ cells
Health sciences
Infertility
Introns
Male
Mammals
Medical research
Meiosis
Mice
Mice, Inbred C57BL
Molecular biology
Polyadenylation
Polyadenylation - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA polymerase
Sequences
Spermatogenesis
Testes
Testis - metabolism
Transcription termination
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The τCstF-64 polyadenylation protein (gene symbol Cstf2t) is a testis-expressed orthologue of CstF-64. Mice in which Cstf2t was knocked out had a phenotype that was only detected in meiotic and postmeiotic male germ cells, giving us the opportunity to examine CstF-64 function in an isolated developmental system. We performed massively parallel clonally amplified sequencing of cDNAs from testes of wild type and Cstf2t(-/-) mice. These results revealed that loss of τCstF-64 resulted in large-scale changes in patterns of genome expression. We determined that there was a significant overrepresentation of RNAs from introns and intergenic regions in testes of Cstf2t(-/-) mice, and a concomitant use of more distal polyadenylation sites. We observed this effect particularly in intronless small genes, many of which are expressed retroposons that likely co-evolved with τCstF-64. Finally, we observed overexpression of long interspersed nuclear element (LINE) sequences in Cstf2t(-/-) testes. These results suggest that τCstF-64 plays a role in 3' end determination and transcription termination for a large range of germ cell-expressed genes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0048373