Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay....

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Published in:PloS one 2012-12, Vol.7 (12), p.e50799-e50799
Main Authors: Palacín, Arantxa, Gómez-Casado, Cristina, Rivas, Luis A, Aguirre, Jacobo, Tordesillas, Leticia, Bartra, Joan, Blanco, Carlos, Carrillo, Teresa, Cuesta-Herranz, Javier, de Frutos, Consolación, Alvarez-Eire, Genoveva García, Fernández, Francisco J, Gamboa, Pedro, Muñoz, Rosa, Sánchez-Monge, Rosa, Sirvent, Sofía, Torres, María J, Varela-Losada, Susana, Rodríguez, Rosalía, Parro, Victor, Blanca, Miguel, Salcedo, Gabriel, Díaz-Perales, Araceli
Format: Article
Language:English
Subjects:
Allergens
Allergens - chemistry
Allergens - immunology
Allergies
Allergy
Al·lèrgens
Analysis
Antigens, Plant - immunology
Care and treatment
Carrier Proteins - immunology
Cloning
Computer Science
Cross Reactions - immunology
Cross-reactivity
Electrophoresis, Polyacrylamide Gel
Epitopes - chemistry
Fertilizer industry
Fertilizers
Food
Food allergies
Food hypersensitivity
Food Hypersensitivity - immunology
Food plants
Fruits
Geography
Humans
Immunoassay
Immunoassay - methods
Immunologia
Immunology
Laboratories
Lipid transfer proteins
Lipids
Lipids - chemistry
Medical research
Medicine
Models, Statistical
Patients
Plant proteins
Plant Proteins - immunology
Pollen
Protein Array Analysis - methods
Protein arrays
Proteins
Proteïnes vegetals
Reactivity
Recombinant Proteins - chemistry
Spain
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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cited_by cdi_FETCH-LOGICAL-c635t-a9b91291358a4f435804c33a2a0e83255cb019f94053485453847a13673f793b3
cites cdi_FETCH-LOGICAL-c635t-a9b91291358a4f435804c33a2a0e83255cb019f94053485453847a13673f793b3
container_end_page e50799
container_issue 12
container_start_page e50799
container_title PloS one
container_volume 7
creator Palacín, Arantxa
Gómez-Casado, Cristina
Rivas, Luis A
Aguirre, Jacobo
Tordesillas, Leticia
Bartra, Joan
Blanco, Carlos
Carrillo, Teresa
Cuesta-Herranz, Javier
de Frutos, Consolación
Alvarez-Eire, Genoveva García
Fernández, Francisco J
Gamboa, Pedro
Muñoz, Rosa
Sánchez-Monge, Rosa
Sirvent, Sofía
Torres, María J
Varela-Losada, Susana
Rodríguez, Rosalía
Parro, Victor
Blanca, Miguel
Salcedo, Gabriel
Díaz-Perales, Araceli
description The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
doi_str_mv 10.1371/journal.pone.0050799
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Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Recercat</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palacín, Arantxa</au><au>Gómez-Casado, Cristina</au><au>Rivas, Luis A</au><au>Aguirre, Jacobo</au><au>Tordesillas, Leticia</au><au>Bartra, Joan</au><au>Blanco, Carlos</au><au>Carrillo, Teresa</au><au>Cuesta-Herranz, Javier</au><au>de Frutos, Consolación</au><au>Alvarez-Eire, Genoveva García</au><au>Fernández, Francisco J</au><au>Gamboa, Pedro</au><au>Muñoz, Rosa</au><au>Sánchez-Monge, Rosa</au><au>Sirvent, Sofía</au><au>Torres, María J</au><au>Varela-Losada, Susana</au><au>Rodríguez, Rosalía</au><au>Parro, Victor</au><au>Blanca, Miguel</au><au>Salcedo, Gabriel</au><au>Díaz-Perales, Araceli</au><au>Stumbles, Phillip A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-12-14</date><risdate>2012</risdate><volume>7</volume><issue>12</issue><spage>e50799</spage><epage>e50799</epage><pages>e50799-e50799</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23272072</pmid><doi>10.1371/journal.pone.0050799</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2012-12, Vol.7 (12), p.e50799-e50799
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1327124928
source PubMed Central Free; Publicly Available Content Database
subjects Allergens
Allergens - chemistry
Allergens - immunology
Allergies
Allergy
Al·lèrgens
Analysis
Antigens, Plant - immunology
Care and treatment
Carrier Proteins - immunology
Cloning
Computer Science
Cross Reactions - immunology
Cross-reactivity
Electrophoresis, Polyacrylamide Gel
Epitopes - chemistry
Fertilizer industry
Fertilizers
Food
Food allergies
Food hypersensitivity
Food Hypersensitivity - immunology
Food plants
Fruits
Geography
Humans
Immunoassay
Immunoassay - methods
Immunologia
Immunology
Laboratories
Lipid transfer proteins
Lipids
Lipids - chemistry
Medical research
Medicine
Models, Statistical
Patients
Plant proteins
Plant Proteins - immunology
Pollen
Protein Array Analysis - methods
Protein arrays
Proteins
Proteïnes vegetals
Reactivity
Recombinant Proteins - chemistry
Spain
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
title Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study
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